Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences.
Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences.
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DOI：
10.1038/s41598-018-35005-2
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发表时间：
2018-11-12
影响因子：
4.6
通讯作者：
Aldridge PD
中科院分区：
文献类型：
--
作者：
Albanna A;Sim M;Hoskisson PA;Gillespie C;Rao CV;Aldridge PD
大肠杆菌和沙门氏菌的鞭毛系统表现出显着水平的遗传和功能同线性。这两个系统都由鞭毛特异性主调节因子FlhD 4C 2控制。从早期鞭毛系统的遗传分析开始，人们就知道E. coliflhDC可与S.肠的。基因组革命已经确定了转录因子和/或DNA结合位点的遗传变化如何影响相关物种的表型结果。因此，我们有兴趣问：使用现代工具来询问鞭毛基因表达和组装，替换S. enterica为E. coli基因在flhDC S.肠道染色体位点？我们发现，即使所有的菌株都是能动的，鞭毛基因的表达是可测量的较低时flhDCEC存在。这些变化可归因于FlhD 4C 2 DNA识别的影响和产生稳定FlhD 4C 2复合物所需的蛋白质-蛋白质相互作用。此外，我们的数据表明，在E. coli中鞭毛系统的FliT调节反馈环的输出有显著差异。我们认为，尽职调查是必要的，在作出假设的基础上异源表达的监管机构，甚至系统显示显着的同线性可能不会表现出完全相同的方式。
The flagellar systems of Escherichia coli and Salmonella enterica exhibit a significant level of genetic and functional synteny. Both systems are controlled by the flagellar specific master regulator FlhD4C2. Since the early days of genetic analyses of flagellar systems it has been known that E. coli flhDC can complement a ∆flhDC mutant in S. enterica. The genomic revolution has identified how genetic changes to transcription factors and/or DNA binding sites can impact the phenotypic outcome across related species. We were therefore interested in asking: using modern tools to interrogate flagellar gene expression and assembly, what would the impact be of replacing the flhDC coding sequences in S. enterica for the E. coli genes at the flhDC S. entercia chromosomal locus? We show that even though all strains created are motile, flagellar gene expression is measurably lower when flhDCEC are present. These changes can be attributed to the impact of FlhD4C2 DNA recognition and the protein-protein interactions required to generate a stable FlhD4C2 complex. Furthermore, our data suggests that in E. coli the internal flagellar FliT regulatory feedback loop has a marked difference with respect to output of the flagellar systems. We argue due diligence is required in making assumptions based on heterologous expression of regulators and that even systems showing significant synteny may not behave in exactly the same manner.
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影响因子：
3.2
作者：
Chubiz, Jessica E. Cott;Golubeva, Yekaterina A.;Slauch, James M.
通讯作者：
Slauch, James M.
影响因子：
4.6
作者：
Sim, Martin;Koirala, Santosh;Aldridge, Phillip D.
通讯作者：
Aldridge, Phillip D.
影响因子：
3.2
作者：
Claret, L;Hughes, C
通讯作者：
Hughes, C
影响因子：
3.7
作者：
Blank K;Hensel M;Gerlach RG
通讯作者：
Gerlach RG
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL