Novel insights into the insect trancriptome response to a natural DNA virus.
Novel insights into the insect trancriptome response to a natural DNA virus.
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关于昆虫转录组对天然 DNA 病毒反应的新见解。
DOI：
10.1186/s12864-015-1499-z
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发表时间：
2015-04-17
期刊：
影响因子：
4.4
通讯作者：
Boots M
中科院分区：
文献类型：
--
作者：
McTaggart SJ;Hannah T;Bridgett S;Garbutt JS;Kaur G;Boots M
人们对无脊椎动物对 DNA 病毒的反应知之甚少。在这里，我们用其天然感染性 DNA 病毒感染了一种具有重要商业价值的害虫 Plodia interpunctella。我们对飞蛾的完整转录组和病毒的部分转录组进行了测序、组装和注释。然后我们测试了暴露于病毒的飞蛾和对照飞蛾之间的基因表达差异。我们发现，与对照组相比，暴露于 DNA 杆状病毒的飞蛾中有 51 个基因存在差异表达。基因集富集分析显示，角质层蛋白在这组基因中显着过高。有趣的是，7 种差异表达的角质层蛋白中有 6 种下调，表明杆状病毒能够操纵宿主的反应。事实上，与对照动物相比，暴露的 51 个基因中还有另外 29 个基因也被下调，其中包括革兰氏阴性结合蛋白。相反，感染后参与转座元件运动的基因上调。我们提出了第一个测量昆虫感染天然 DNA 病毒后全基因组基因表达的实验。我们的结果表明角质层蛋白可能是支持 DNA 病毒反应的关键基因。此外，病毒暴露后大部分基因下调表明该病毒正在积极操纵昆虫免疫反应。最后，转座元件活性似乎在病毒入侵期间可能会增加。综合起来，这些结果提供了急需的宿主候选基因，可以对 DNA 病毒入侵者做出反应。
Little is known about invertebrate responses to DNA viruses. Here, we infect a commercially important pest moth species Plodia interpunctella with its naturally infecting DNA virus. We sequenced, assembled and annotated the complete transcriptome of the moth, and a partial transcriptome of the virus. We then tested for differential gene expression between moths that were exposed to the virus and controls. We found 51 genes that were differentially expressed in moths exposed to a DNA baculovirus compared to controls. Gene set enrichment analysis revealed that cuticle proteins were significantly overrepresented in this group of genes. Interestingly, 6 of the 7 differentially expressed cuticle proteins were downregulated, suggesting that baculoviruses are able to manipulate its host’s response. In fact, an additional 29 of the 51 genes were also downregulated in exposed compared with control animals, including a gram-negative binding protein. In contrast, genes involved in transposable element movement were upregulated after infection. We present the first experiment to measure genome-wide gene expression in an insect after infection with a natural DNA virus. Our results indicate that cuticle proteins might be key genes underpinning the response to DNA viruses. Furthermore, the large proportion of genes that were downregulated after viral exposure suggests that this virus is actively manipulating the insect immune response. Finally, it appears that transposable element activity might increase during viral invasion. Combined, these results provide much needed host candidate genes that respond to DNA viral invaders.
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影响因子：
5.6
作者：
Kingsolver MB;Huang Z;Hardy RW
通讯作者：
Hardy RW
影响因子：
12.3
作者：
Gerardo NM;Altincicek B;Anselme C;Atamian H;Barribeau SM;de Vos M;Duncan EJ;Evans JD;Gabaldón T;Ghanim M;Heddi A;Kaloshian I;Latorre A;Moya A;Nakabachi A;Parker BJ;Pérez-Brocal V;Pignatelli M;Rahbé Y;Ramsey JS;Spragg CJ;Tamames J;Tamarit D;Tamborindeguy C;Vincent-Monegat C;Vilcinskas A
通讯作者：
Vilcinskas A
影响因子：
30.5
作者：
Goic, Bertsy;Vodovar, Nicolas;Saleh, Maria-Carla
通讯作者：
Saleh, Maria-Carla
影响因子：
3
作者：
Jones M;Blaxter M
通讯作者：
Blaxter M
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.