Multiple Copies of a Simple MYB-Binding Site Confers Trans-regulation by Specific Flavonoid-Related R2R3 MYBs in Diverse Species.
Multiple Copies of a Simple MYB-Binding Site Confers Trans-regulation by Specific Flavonoid-Related R2R3 MYBs in Diverse Species.
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DOI：
10.3389/fpls.2017.01864
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发表时间：
2017
影响因子：
5.6
通讯作者：
Allan AC
中科院分区：
文献类型：
--
作者：
Brendolise C;Espley RV;Lin-Wang K;Laing W;Peng Y;McGhie T;Dejnoprat S;Tomes S;Hellens RP;Allan AC
在苹果中，MYB转录因子MYB10控制花青素的积累。MYB10能够通过将自己的启动子与特定的基序R1基序结合来自动激活其表达。在一些苹果材料中，一个被称为R6的自然突变在MYB10启动子中具有更多的这个基序的拷贝，导致更强的自动激活和花青素的增加。在这里，我们展示了从苹果、梨、草莓、矮牵牛、猕猴桃和拟南芥中选择的其他花青素相关的MYB能够激活含有R6基序的启动子。为了检验这个基序的特异性，对R2R3MYB家族的成员进行了筛选，以对抗含有R6突变的启动子。只有亚组5和亚组6的MYB通过与R6基序结合来激活表达，这些MYB在其R2R3 DNA结合区共享保守残基。将苹果R6基序插入到梨(PcMYB10)和拟南芥(AtMY75)MYB10的同源启动子中，提高了花青素的水平。将R6基序导入猕猴桃花青素生物合成酶F3‘5’H的启动子区域，可实现MYB10的调控。这导致烟草和猕猴桃中的飞雀素水平升高。最后，R6基序插入到维生素C生物合成基因Gdp-L-Gal磷酸化酶基因中，以MYB10依赖的方式增加维生素C含量。因此，这个基序提供了一个工具来重新设计植物中新的MYB调节的反应。
In apple, the MYB transcription factor MYB10 controls the accumulation of anthocyanins. MYB10 is able to auto-activate its expression by binding its own promoter at a specific motif, the R1 motif. In some apple accessions a natural mutation, termed R6, has more copies of this motif within the MYB10 promoter resulting in stronger auto-activation and elevated anthocyanins. Here we show that other anthocyanin-related MYBs selected from apple, pear, strawberry, petunia, kiwifruit and Arabidopsis are able to activate promoters containing the R6 motif. To examine the specificity of this motif, members of the R2R3 MYB family were screened against a promoter harboring the R6 mutation. Only MYBs from subgroups 5 and 6 activate expression by binding the R6 motif, with these MYBs sharing conserved residues in their R2R3 DNA binding domains. Insertion of the apple R6 motif into orthologous promoters of MYB10 in pear (PcMYB10) and Arabidopsis (AtMY75) elevated anthocyanin levels. Introduction of the R6 motif into the promoter region of an anthocyanin biosynthetic enzyme F3′5′H of kiwifruit imparts regulation by MYB10. This results in elevated levels of delphinidin in both tobacco and kiwifruit. Finally, an R6 motif inserted into the promoter the vitamin C biosynthesis gene GDP-L-Gal phosphorylase increases vitamin C content in a MYB10-dependent manner. This motif therefore provides a tool to re-engineer novel MYB-regulated responses in plants.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
DOI：
10.1051/agro:19880806
发表时间：
1988-01-01
期刊：
AGRONOMIE
影响因子：
--
作者：
BRISSET, MN;PAULIN, JP;DURON, M
通讯作者：
DURON, M
影响因子：
7.2
作者：
Dubos, Christian;Le Gourrierec, Jose;Lepiniec, Loic
通讯作者：
Lepiniec, Loic
影响因子：
4.2
作者：
Espley, Richard V.;Butts, Christine A.;Hellens, Roger P.
通讯作者：
Hellens, Roger P.
影响因子：
11.6
作者：
Albert, Nick W.;Davies, Kevin M.;Schwinn, Kathy E.
通讯作者：
Schwinn, Kathy E.