The Nucleocapsid protein triggers the main humoral immune response in COVID-19 patients.
The Nucleocapsid protein triggers the main humoral immune response in COVID-19 patients.
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DOI：
10.1016/j.bbrc.2021.01.073
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发表时间：
2021-03-05
影响因子：
3.1
通讯作者：
Freire R
中科院分区：
文献类型：
--
作者：
Smits VAJ;Hernández-Carralero E;Paz-Cabrera MC;Cabrera E;Hernández-Reyes Y;Hernández-Fernaud JR;Gillespie DA;Salido E;Hernández-Porto M;Freire R
为了控制由SARS-CoV-2感染引起的新冠肺炎大流行，在识别感染患者和检测对该病毒的免疫反应阳性的患者方面取得了重大进展。目前，研制针对冠状病毒的疫苗的努力正在进行中。为了了解SARS-CoV-2的免疫反应性，我们用重组病毒颗粒蛋白N(核衣壳)、M(膜)和S(Spike)，用斑点印迹法比较了感染患者和对照患者对SARS-CoV-2的抗体应答。此外，我们使用N蛋白和S蛋白的不同蛋白片段来绘制免疫表位图。大多数新冠肺炎患者对N蛋白的全长和片段产生特异性免疫反应，少数患者对S蛋白的第300-685位氨基酸片段产生特异性免疫应答。相反，对其他S蛋白片段或M蛋白的免疫反应性较低。这种反应是新冠肺炎患者特有的，因为很少的对照组患者表现出免疫反应，可能反映了对其他冠状病毒的免疫反应。总之，我们的结果可能有助于发展新冠肺炎抗体反应的检测方法(S)，检测此类SARS-CoV-2抗体的方法的选择性和疫苗的开发。
In order to control the COVID-19 pandemic caused by SARS-CoV-2 infection, serious progress has been made to identify infected patients and to detect patients with a positive immune response against the virus. Currently, attempts to generate a vaccine against the coronavirus are ongoing. To understand SARS-CoV-2 immunoreactivity, we compared the IgG antibody response against SARS-CoV-2 in infected versus control patients by dot blot using recombinant viral particle proteins: N (Nucleocapsid), M (Membrane) and S (Spike). In addition, we used different protein fragments of the N and S protein to map immune epitopes. Most of the COVID-19 patients presented a specific immune response against the full length and fragments of the N protein and, to lesser extent, against a fragment containing amino acids 300–685 of the S protein. In contrast, immunoreactivity against other S protein fragments or the M protein was low. This response is specific for COVID-19 patients as very few of the control patients displayed immunoreactivity, likely reflecting an immune response against other coronaviruses. Altogether, our results may help develop method(s) for measuring COVID-19 antibody response, selectivity of methods detecting such SARS-CoV-2 antibodies and vaccine development.
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DOI：
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发表时间：
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影响因子：
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作者：
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