Gene expression evolution in pattern-triggered immunity within Arabidopsis thaliana and across Brassicaceae species.
Gene expression evolution in pattern-triggered immunity within Arabidopsis thaliana and across Brassicaceae species.
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DOI：
10.1093/plcell/koab073
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发表时间：
2021-07-19
期刊：
影响因子：
--
通讯作者：
Tsuda K
中科院分区：
文献类型：
--
作者：
Winkelmüller TM;Entila F;Anver S;Piasecka A;Song B;Dahms E;Sakakibara H;Gan X;Kułak K;Sawikowska A;Krajewski P;Tsiantis M;Garrido-Oter R;Fukushima K;Schulze-Lefert P;Laurent S;Bednarek P;Tsuda K
植物通过感知微生物相关分子模式（MAMPs）来识别周围的微生物，从而激活模式触发免疫（PTI）。尽管它们对微生物控制的重要性，PTI反应的演变在很大程度上仍然没有表征。在这里，通过采用比较转录组学的6个拟南芥加入和3个额外的拟南芥科物种调查PTI的反应，我们确定了一组基因，通常响应的MAMP flg22和基因，表现出物种特异性的表达签名。flg22触发的转录组反应在菊科物种之间的变化与它们的遗传不一致，而表达变化在A. thaliana.我们发现WRKY转录因子结合位点在保守和物种特异性应答基因的5′调控区富集，将WRKY结合位点的出现与PTI期间基因表达模式的进化联系起来。我们的研究结果推进了我们对生物胁迫期间转录组进化的理解。密切相关的菊科植物在模式触发免疫过程中的基因表达模式显示出相当大的变化。
Plants recognize surrounding microbes by sensing microbe-associated molecular patterns (MAMPs) to activate pattern-triggered immunity (PTI). Despite their significance for microbial control, the evolution of PTI responses remains largely uncharacterized. Here, by employing comparative transcriptomics of six Arabidopsis thaliana accessions and three additional Brassicaceae species to investigate PTI responses, we identified a set of genes that commonly respond to the MAMP flg22 and genes that exhibit species-specific expression signatures. Variation in flg22-triggered transcriptome responses across Brassicaceae species was incongruent with their phylogeny, while expression changes were strongly conserved within A. thaliana. We found the enrichment of WRKY transcription factor binding sites in the 5′-regulatory regions of conserved and species-specific responsive genes, linking the emergence of WRKY-binding sites with the evolution of gene expression patterns during PTI. Our findings advance our understanding of the evolution of the transcriptome during biotic stress. Closely related Brassicaceae plants show considerable variation in gene expression patterns during pattern-triggered immunity.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
10.7
作者：
Dean R;Harrison PW;Wright AE;Zimmer F;Mank JE
通讯作者：
Mank JE
影响因子：
7.2
作者：
Coolen, Silvia;Proietti, Silvia;Van Wees, Saskia C. M.
通讯作者：
Van Wees, Saskia C. M.
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y