Starvation during pregnancy impairs fetal oogenesis and folliculogenesis in offspring in the mouse.
Starvation during pregnancy impairs fetal oogenesis and folliculogenesis in offspring in the mouse.
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怀孕期间的饥饿会损害小鼠后代的胎儿卵子发生和卵泡发生
DOI：
10.1038/s41419-018-0492-2
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发表时间：
2018-05-01
影响因子：
9
通讯作者：
Shen W
中科院分区：
文献类型：
--
作者：
Wang JJ;Yu XW;Wu RY;Sun XF;Cheng SF;Ge W;Liu JC;Li YP;Liu J;Zou SH;De Felici M;Shen W
虽然越来越明显的是，怀孕期间的母体饥饿会对后代的一系列生理过程产生永久性的影响，但关于这种状况对卵子发生的影响，从而对哺乳动物后代的终生繁殖成功的影响，我们知之甚少。在本研究中，我们通过在卵巢分化时(性交后12.5天)连续三天饥饿怀孕的小鼠来解决这个问题，并首先分析了饥饿对胚胎卵母细胞的存活及其在减数分裂前期I(MPI)阶段的进展能力的影响，然后分析了对后代出生后卵泡发生的影响。结果表明，母体饥饿增加了胎儿卵巢细胞的凋亡率，导致卵母细胞数量减少。此外，存活的卵母细胞的MPI进程被减缓，饥饿的卵巢中DNA修复因子的表达增加。转录组分析确定了61个在对照卵巢和饥饿卵巢之间差异表达的基因，其中大部分参与了代谢过程。在产后3天(DPP)饥饿的卵巢中，被原始卵泡包裹的卵母细胞百分率和与卵泡发生密切相关的卵母细胞基因Nobox、Lhx8和Sohlh2的表达显著下降。最后，在幼年期(21DPP)，饥饿母亲的子代卵巢中的卵母细胞数和有腔卵泡数显著低于对照组。我们的发现支持这样一种观点，即母亲饥饿会影响后代的卵巢发育，这可能会对他们成年后的生殖成功产生不利影响。
Although it is becoming increasingly evident that maternal starvation during pregnancy can have permanent effects on a range of physiological processes in the offspring, scant information is available about the consequence of such condition for oogenesis and hence for lifetime reproductive success of progeny in mammals. In the present study, we address this topic by starving pregnant mice at the time of ovarian differentiation (12.5 days post coitum (dpc)) for three consecutive days and analyzed the consequence first on the survival of the fetal oocytes and their capability to progress throughout the stages of meiotic prophase I (MPI) and then on the postnatal folliculogenesis of the offspring. The results showed that maternal starvation increased apoptosis in the fetal ovaries, resulting in reduction of the oocyte number. Moreover, MPI progression was slowed down in the surviving oocytes and the expression of DNA repair players in the starved ovaries increased. Transcriptome analysis identified 61 differentially expressed genes between control and starved ovaries, the most part of these being involved in metabolic processes. A significant decrease in the percentage of oocytes enclosed in primordial follicles and the expression of oocyte genes critically involved in folliculogenesis such as Nobox, Lhx8 and Sohlh2 in the 3 days post partum (dpp) starved ovaries were found. Finally, at the time of juvenile period (21 dpp), the number of oocytes and antral follicles resulted significantly lower in the ovaries of the offspring from starved mothers in comparison to controls. Our findings support the notion that maternal starvation can affect ovary development in the offspring that could adversely affect their reproductive success in the adult life.
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影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
影响因子：
2.5
作者：
de Bruin, JP;Dorland, M;Te Velde, ER
通讯作者：
Te Velde, ER
DOI：
10.1111/j.1471-0528.2008.01822.x
发表时间：
2008-09-01
影响因子：
5.8
作者：
Painter, R. C.;Osmond, C.;Roseboom, T. J.
通讯作者：
Roseboom, T. J.
影响因子：
2.8
作者：
Kang, Bo;Jiang, Dong Mei;Ma, Rong
通讯作者：
Ma, Rong
影响因子：
4
作者：
De Felici, M;Klinger, FG;Lobascio, M
通讯作者：
Lobascio, M