N-acetylpenicillamine inhibits the replication of porcine reproductive and respiratory syndrome virus in vitro.
N-acetylpenicillamine inhibits the replication of porcine reproductive and respiratory syndrome virus in vitro.
复制标题
N-乙酰青霉胺体外抑制猪繁殖与呼吸综合征病毒的复制
DOI：
10.1007/s11259-010-9435-9
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发表时间：
2010-10
影响因子：
2.2
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Jiang Y;Fang L;Luo R;Xiao S;Chen H
一氧化氮（NO）被认为是对抗某些微生物的重要分子。在本研究中，我们研究了NO在体外对猪繁殖与呼吸综合征病毒（PRRSV）感染的抑制作用以及NO在防御PRRSV中的作用。我们的结果表明，外源性 NO 不能抑制 PRRSV 感染。出乎意料的是，N-乙酰青霉胺 (NAP)（一种常用的作为 NO 产生试剂阴性对照的化合物）能够抑制 PRRSV 复制。因此，进一步探讨了NAP对PRRSV复制的抑制作用。我们发现，NAP 对 PRRSV 复制的最大抑制作用是在病毒感染后 1 小时进行处理时实现的，并且在 400 μM NAP 存在的情况下，病毒产量减少了约 50 倍。对病毒RNA和蛋白质合成也有明显的抑制作用。然而，仅在病毒感染的早期阶段才发挥抑制作用。取消 NAP 处理后，病毒产量可以恢复正常。抑制作用可能是由NAP的巯基还原能力和金属螯合特性引起的。这些研究表明 (i) NO 产生或 NO 合酶 (NOS) 表达谱可能不是 PRRSV 免疫反应的可靠指标； (ii) NAP可以抑制PRRSV的复制。
Nitric oxide (NO) was proposed to be an important molecule against some microorganisms. In this study, we investigated the inhibitory effect of NO on the infection by porcine reproductive and respiratory syndrome virus (PRRSV) in vitro and the role of NO in the defense against PRRSV. Our results indicated that exogenous NO did not inhibit PRRSV infection. Unexpectedly, N-acetylpenicillamine (NAP), a commonly used compound as negative control for NO-producing reagents, inhibited PRRSV replication. Thus, the inhibition effect of NAP on PRRSV replication was further explored. We found that the maximal inhibition effect of NAP on PRRSV replication was achieved upon treatment 1 h after virus infection and the virus yield was reduced by approximately 50 fold in the presence of 400 μM NAP. An obvious inhibitory effect on viral RNA and protein synthesis was also observed. However, the inhibitory effect was only achieved at early phase of virus infection. The normal virus yield could be restored upon the removal of NAP treatment. The inhibitory effect might be caused by sulfhydryl-reducing capacity and metal chelating properties of NAP. These studies suggested that (i) NO production or NO synthase (NOS) expression profiling may not be a reliable index for the immune response to PRRSV; (ii) NAP could inhibit the replication of PRRSV.
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影响因子：
1.5
作者：
BENFIELD, DA;NELSON, E;CHLADEK, D
通讯作者：
CHLADEK, D
影响因子：
1.8
作者：
Jung K;Gurnani A;Renukaradhya GJ;Saif LJ
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影响因子：
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作者：
Åkerström, S;Mousavi-Jazi, M;Mirazimi, A
通讯作者：
Mirazimi, A
影响因子：
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作者：
BI, ZB;REISS, CS
通讯作者：
REISS, CS
影响因子：
5.4
作者：
Lin, YL;Huang, YL;Liao, CL
通讯作者：
Liao, CL