MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability.
MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability.
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DOI：
10.1016/j.devcel.2017.09.024
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发表时间：
2017-12-04
影响因子：
11.8
通讯作者：
Zhu JK
中科院分区：
文献类型：
--
作者：
Zhao C;Wang P;Si T;Hsu CC;Wang L;Zayed O;Yu Z;Zhu Y;Dong J;Tao WA;Zhu JK
丝裂原活化蛋白激酶级联是将环境刺激转化为细胞反应的重要信号模块。我们发现，MPK 3，MPK 4和MPK 6在冷处理后迅速激活。mpk 3和mpk 6突变体显示CBF基因表达增加和抗冻性增强，而植物中MKK 4/5-MPK 3/6级联的组成性激活导致CBF基因表达减少和对冷冻的超敏反应，表明MKK 4/5-MPK 3/6级联负调控冷反应。MPK 3和MPK 6可以磷酸化调节CBF基因表达的bHLH转录因子ICE 1，磷酸化促进ICE 1的降解。有趣的是，MEKK 1-MKK 2-MPK 4通路组成性地抑制MPK 3和MPK 6活性，并且在冷反应中具有积极作用。此外，MAPKKK YDA和两种钙/钙调蛋白调节的受体样激酶CRLK 1和CRLK 2负调节MPK 3/6的冷激活。我们的研究结果揭示了MAPK级联在植物冷反应调控中的重要作用。ICE 1是植物冷反应的中央调节器，其水平受到严格控制。Zhao等人表明，冷激活的MPK 3和MPK 6磷酸化ICE 1并促进其降解，从而负调节冷反应，而MPK 4通过组成性抑制MPK 3和MPK 6活性正调节冷反应。
Mitogen-activated protein kinase cascades are important signaling modules that convert environmental stimuli into cellular responses. We show that MPK3, MPK4, and MPK6 are rapidly activated after cold treatment. The mpk3 and mpk6 mutants display increased expression of CBF genes and enhanced freezing tolerance, whereas constitutive activation of the MKK4/5-MPK3/6 cascade in plants causes reduced expression of CBF genes and hypersensitivity to freezing, suggesting that the MKK4/5-MPK3/6 cascade negatively regulates the cold response. MPK3 and MPK6 can phosphorylate ICE1, a bHLH transcription factor that regulates the expression of CBF genes, and the phosphorylation promotes the degradation of ICE1. Interestingly, the MEKK1-MKK2-MPK4 pathway constitutively suppresses MPK3 and MPK6 activities, and has a positive role in the cold response. Furthermore, the MAPKKK YDA, and two calcium/calmodulin-regulated receptor-like kinases, CRLK1 and CRLK2, negatively modulate the cold-activation of MPK3/6. Our results uncover important roles of MAPK cascades in the regulation of plant cold response. ICE1 is a central regulator of the plant cold response and its levels are tightly controlled. Zhao et al. show that cold-activated MPK3 and MPK6 phosphorylate ICE1 and promote its degradation, thus negatively regulating the cold response, whereas MPK4 positively regulates the cold response by constitutively suppressing MPK3 and MPK6 activity.
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