Methods To Assess Shear-Thinning Hydrogels for Application As Injectable Biomaterials.
Methods To Assess Shear-Thinning Hydrogels for Application As Injectable Biomaterials.
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DOI：
10.1021/acsbiomaterials.7b00734
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发表时间：
2017-12-11
影响因子：
5.8
通讯作者：
Burdick JA
中科院分区：
文献类型：
--
作者：
Chen MH;Wang LL;Chung JJ;Kim YH;Atluri P;Burdick JA
可注射水凝胶作为用于递送细胞、生长因子和其他分子以定位和改善它们在注射部位的保留以及用于组织的机械膨胀的媒介物已经得到普及。然而，当评估水凝胶用于此类应用时，需要考虑许多因素，例如粘度、储存和损耗模量以及注射力。现在有许多工具可用于定量评估这些因素，包括剪切稀化水凝胶，因为它们的性质在机械负荷下会发生变化。在这里，我们描述了相关的流变学测试和方法来测量注射力，使用力传感器或机械测试机对可注射水凝胶的评价。可注射的剪切致稀水凝胶可用于多种临床应用，例如，我们重点关注注射到心脏的方法，其中了解注射特性和机械力对于水凝胶的一致递送和保留至关重要。我们讨论了在心肌梗死模型中将水凝胶输送到小鼠、大鼠和猪心脏的方法，并比较了水凝胶保存的组织后处理方法。我们的目的是，本文所述的方法可以有助于设计和评估广泛的生物医学应用的剪切稀化水凝胶。
Injectable hydrogels have gained popularity as a vehicle for the delivery of cells, growth factors, and other molecules to localize and improve their retention at the injection site, as well as for the mechanical bulking of tissues. However, there are many factors, such as viscosity, storage and loss moduli, and injection force, to consider when evaluating hydrogels for such applications. There are now numerous tools that can be used to quantitatively assess these factors, including for shear-thinning hydrogels because their properties change under mechanical load. Here, we describe relevant rheological tests and ways to measure injection force using a force sensor or a mechanical testing machine toward the evaluation of injectable hydrogels. Injectable, shear-thinning hydrogels can be used in a variety of clinical applications, and as an example we focus on methods for injection into the heart, where an understanding of injection properties and mechanical forces is imperative for consistent hydrogel delivery and retention. We discuss methods for delivery of hydrogels to mouse, rat, and pig hearts in models of myocardial infarction, and compare methods of tissue postprocessing for hydrogel preservation. Our intent is that the methods described herein can be helpful in the design and assessment of shear-thinning hydrogels for widespread biomedical applications.
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影响因子：
6.2
作者：
Bakota EL;Sensoy O;Ozgur B;Sayar M;Hartgerink JD
通讯作者：
Hartgerink JD
DOI：
10.1002/adma.201501099
发表时间：
2015-07-15
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Hong S;Sycks D;Chan HF;Lin S;Lopez GP;Guilak F;Leong KW;Zhao X
通讯作者：
Zhao X
DOI：
10.1073/pnas.0701980104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Haines-Butterick, Lisa;Rajagopal, Karthikan;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
影响因子：
14
作者：
Gupta, D;Tator, CH;Shoichet, MS
通讯作者：
Shoichet, MS
影响因子：
20.1
作者：
Gao E;Lei YH;Shang X;Huang ZM;Zuo L;Boucher M;Fan Q;Chuprun JK;Ma XL;Koch WJ
通讯作者：
Koch WJ