A comparative study on the interactions of SMAP-29 with lipid monolayers.
A comparative study on the interactions of SMAP-29 with lipid monolayers.
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DOI：
10.1016/j.bbamem.2009.09.017
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发表时间：
2010-05
影响因子：
3.4
通讯作者：
Gidalevitz, David
中科院分区：
文献类型：
--
作者：
Neville, Frances;Ivankin, Andrey;Konovalov, Oleg;Gidalevitz, David
这项工作研究了绵羊抗菌肽SMAP-29对脂质单层的区分，并将其与人LL-37肽进行了比较。在朗缪尔槽中形成流体磷脂单分子膜，随后用X射线反射率和掠入射X射线衍射的X射线散射技术进行了研究。在单层下注射肽后磷脂结构的任何变化被认为是由于肽和脂质之间的相互作用。数据显示SMAP-29以与LL-37类似的方式区分带负电荷的磷脂。然而，更有趣的是注意到，尽管SMAP-29在单层附近的浓度更高，但与LL-37相比，其更大的电荷确保了SMAP-29的浓度，观察单层破坏所需的SMAP-29的量是用于观察相同系统的类似变化的LL-37分子数量的约3.5倍。这一结果表明，结构，氨基酸序列或大小的肽可能是一样重要的电荷，因此提供了许多关于新的药物设计和开发的抗菌肽的进一步研究的影响。
This work investigates the discrimination of lipid monolayers by the ovine antimicrobial peptide SMAP-29 and compares it to that of the human LL-37 peptide. Fluid phospholipid monolayers were formed in a Langmuir trough and subsequently studied with the X-ray scattering techniques of X-ray reflectivity and grazing incidence X-ray diffraction. Any changes in the phospholipid structure after injection of peptide under the monolayer were considered to be due to interactions between the peptides and lipids. The data show that SMAP-29 discriminates against negatively charged phospholipids in a similar way to LL-37. However, it is even more interesting to note that despite a higher concentration of SMAP-29 near the monolayer, ensured by its greater charge as compared to LL-37, the amount of SMAP-29 needed to observe monolayer disruption was around three and a half times the number of molecules of LL-37 used to see similar changes with the same system. This result suggests that the structure, amino acid sequence or size of the peptide may well be as important as electrical charge and therefore gives many implications for the further study of antimicrobial peptides with regards to novel drug design and development.
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DOI：
10.1016/0005-2736(75)90045-0
发表时间：
1975-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
DEMEL, RA;GEURTSVANKESSEL, WSM;VANDEENEN, LLM
通讯作者：
VANDEENEN, LLM
影响因子：
3.5
作者：
BAGELLA, L;SCOCCHI, M;ZANETTI, M
通讯作者：
ZANETTI, M
影响因子：
2.8
作者：
Konovalov, O;O'Flaherty, SM;Lohner, K
通讯作者：
Lohner, K
影响因子：
2.9
作者：
Henzler-Wildman, KA;Martinez, GV;Ramamoorthy, A
通讯作者：
Ramamoorthy, A
DOI：
10.1111/j.1432-1033.1996.0325z.x
发表时间：
1996-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Gudmundsson, GH;Agerberth, B;Salcedo, R
通讯作者：
Salcedo, R