Biochemical and transcriptome analyses of a novel chlorophyll-deficient chlorina tea plant cultivar.
Biochemical and transcriptome analyses of a novel chlorophyll-deficient chlorina tea plant cultivar.
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DOI：
10.1186/s12870-014-0352-x
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发表时间：
2014-12-10
影响因子：
5.3
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang L;Yue C;Cao H;Zhou Y;Zeng J;Yang Y;Wang X
茶树（Camellia sinensis（L.）O. Kuntze）是最重要的经济木本作物之一。近年来，在茶树育种过程中培育出了许多叶色基因型，成为绿色茶加工的重要材料。虽然一些茶树叶色突变体的生理特性已经部分揭示，但对导致茶树叶绿素表型的分子机制知之甚少。中黄2号（ZH 2）是在茶树育种过程中选育出的黄叶茶树新品种。与广泛种植的绿色茶树品种龙井43（LJ 43）相比，ZH 2表现出叶绿素表型，叶绿素含量显著降低。透射电子显微镜分析表明，ZH 2叶绿体的超微结构被破坏，基粒堆积不良。ZH 2的茶氨酸和游离氨基酸含量显著高于LJ 43，而类胡萝卜素、儿茶素和花青素含量显著低于LJ 43。基因芯片分析结果表明，与LJ 43相比，ZH 2地上部中与氨基酸代谢、光合作用和色素代谢相关的259个基因的表达量发生了显著变化。对4，902个差异表达基因的通路分析确定了24条通路受到显著调节，包括“半胱氨酸和甲硫氨酸代谢”、“甘氨酸、丝氨酸和苏氨酸代谢”、“类黄酮生物合成”、“卟啉和叶绿素代谢”和“类胡萝卜素生物合成”。此外，一些差异表达的基因可以定位到“茶氨酸生物合成”，“叶绿素生物合成”和“类黄酮生物合成”途径。参与这些途径的基因表达的变化可能是ZH 2不同表型的原因。鉴定了一种新的叶绿素缺乏型绿球藻茶树品种。生化特性进行了分析，基因表达谱进行了使用定制的基于谷胱甘肽的微阵列。本研究提供了进一步的见解的分子机制的表型的chlorina品种的茶树。本文的在线版本（doi：10.1186/s12870-014-0352-x）包含补充材料，可供授权用户使用。
The tea plant (Camellia sinensis (L.) O. Kuntze) is one of the most economically important woody crops. Recently, many leaf color genotypes have been developed during tea plant breeding and have become valuable materials in the processing of green tea. Although the physiological characteristics of some leaf color mutants of tea plants have been partially revealed, little is known about the molecular mechanisms leading to the chlorina phenotype in tea plants. The yellow-leaf tea cultivar Zhonghuang 2 (ZH2) was selected during tea plant breeding. In comparison with Longjing 43 (LJ43), a widely planted green tea cultivar, ZH2 exhibited the chlorina phenotype and displayed significantly decreased chlorophyll contents. Transmission electron microscopy analysis revealed that the ultrastructure of the chloroplasts was disrupted, and the grana were poorly stacked in ZH2. Moreover, the contents of theanine and free amino acids were significantly higher, whereas the contents of carotenoids, catechins and anthocyanin were lower in ZH2 than in LJ43. Microarray analysis showed that the expression of 259 genes related to amino acid metabolism, photosynthesis and pigment metabolism was significantly altered in ZH2 shoots compared with those of LJ43 plants. Pathway analysis of 4,902 differentially expressed genes identified 24 pathways as being significantly regulated, including ‘cysteine and methionine metabolism’, ‘glycine, serine and threonine metabolism’, ‘flavonoid biosynthesis’, ‘porphyrin and chlorophyll metabolism’ and ‘carotenoid biosynthesis’. Furthermore, a number of differentially expressed genes could be mapped to the ‘theanine biosynthesis’, ‘chlorophyll biosynthesis’ and ‘flavonoid biosynthesis’ pathways. Changes in the expression of genes involved in these pathways might be responsible for the different phenotype of ZH2. A novel chlorophyll-deficient chlorina tea plant cultivar was identified. Biochemical characteristics were analyzed and gene expression profiling was performed using a custom oligonucleotide-based microarray. This study provides further insights into the molecular mechanisms underlying the phenotype of the chlorina cultivar of Camellia sinensis. The online version of this article (doi:10.1186/s12870-014-0352-x) contains supplementary material, which is available to authorized users.
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影响因子：
10.7
作者：
Kidron, Heidi;Repo, Susanna;Salminen, Tiina A.
通讯作者：
Salminen, Tiina A.
DOI：
10.1007/s004380050394
发表时间：
1997-03-18
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Kannangara, CG;Vothknecht, UC;von Wettstein, D
通讯作者：
von Wettstein, D
影响因子：
7.3
作者：
Howitt, CA;Pogson, BJ
通讯作者：
Pogson, BJ
影响因子：
15.3
作者：
Juneja, LR;Chu, DC;Yokogoshi, H
通讯作者：
Yokogoshi, H
影响因子：
2
作者：
Chen Jin, Chen Jin;Wang PingSheng, Wang PingSheng;Pei, S. J.
通讯作者：
Pei, S. J.