Chitosan primes plant defence mechanisms against Botrytis cinerea, including expression of Avr9/Cf-9 rapidly elicited genes.
Chitosan primes plant defence mechanisms against Botrytis cinerea, including expression of Avr9/Cf-9 rapidly elicited genes.
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DOI：
10.1111/pce.13921
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Newton A
中科院分区：
文献类型：
--
作者：
De Vega D;Holden N;Hedley PE;Morris J;Luna E;Newton A
目前针对真菌病原体灰葡萄孢的作物保护策略依赖于常规杀真菌剂和宿主遗传抗性的组合。然而，由于病原体的进化和杀真菌剂使用的立法，这些策略不足以保护植物免受这种病原体的侵害。防御激发子可以通过一种称为防御启动的现象刺激植物防御机制。引发导致在宿主识别病原体时更快和/或更强的抗性表达。这项工作的目的是研究防御引发的商业配方的诱导剂壳聚糖。用壳聚糖处理导致茄科和十字花科植物的诱导抗性（IR）。在番茄植物中，增强的抗性与胼胝质沉积的引发和植物激素茉莉酸（JA）的积累有关。大规模转录组学分析显示，壳聚糖在感染后的早期时间点引发基因表达。此外，还鉴定了两个具有特征性引发谱的新番茄基因，Avr 9/Cf-9快速引发蛋白75（ACRE 75）和180（ACRE 180）。ACRE 75、ACRE 180及其本氏烟草同源物的瞬时和稳定过表达揭示了它们是植物对B抗性的正调节剂。灰叶这为寻找保护茄科植物免受B侵害的策略提供了有价值的信息。灰叶壳聚糖引发细胞壁防御、防御相关激素的积累和Avr 9/Cf-9的表达快速引发番茄中抗灰葡萄孢的基因，这为寻找茄科作物的保护策略提供了有价值的信息。
Current crop protection strategies against the fungal pathogen Botrytis cinerea rely on a combination of conventional fungicides and host genetic resistance. However, due to pathogen evolution and legislation in the use of fungicides, these strategies are not sufficient to protect plants against this pathogen. Defence elicitors can stimulate plant defence mechanisms through a phenomenon known as defence priming. Priming results in a faster and/or stronger expression of resistance upon pathogen recognition by the host. This work aims to study defence priming by a commercial formulation of the elicitor chitosan. Treatments with chitosan result in induced resistance (IR) in solanaceous and brassicaceous plants. In tomato plants, enhanced resistance has been linked with priming of callose deposition and accumulation of the plant hormone jasmonic acid (JA). Large‐scale transcriptomic analysis revealed that chitosan primes gene expression at early time‐points after infection. In addition, two novel tomato genes with a characteristic priming profile were identified, Avr9/Cf‐9 rapidly elicited protein 75 (ACRE75) and 180 (ACRE180). Transient and stable over‐expression of ACRE75, ACRE180 and their Nicotiana benthamiana homologs, revealed that they are positive regulators of plant resistance against B. cinerea. This provides valuable information in the search for strategies to protect Solanaceae plants against B. cinerea. Chitosan primes cell wall defences, accumulation of defence‐related hormones and expression of Avr9/Cf‐9 rapidly elicited genes against Botrytis cinerea in Solanum lycopersicum (tomato), which provides valuable information in the search for protection strategies in Solanaceous crops.
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影响因子：
3.7
作者：
Moffat CS;Ingle RA;Wathugala DL;Saunders NJ;Knight H;Knight MR
通讯作者：
Knight MR
影响因子：
5.6
作者：
Aranega-Bou P;de la O Leyva M;Finiti I;García-Agustín P;González-Bosch C
通讯作者：
González-Bosch C
影响因子：
--
作者：
Luna, E.
通讯作者：
Luna, E.
DOI：
10.1590/s0104-14282009000300013
发表时间：
2009-01-01
期刊：
Polímeros
影响因子：
--
作者：
Goy, Rejane C.;Britto, Douglas de;Assis, Odilio B. G.
通讯作者：
Assis, Odilio B. G.
影响因子：
7.4
作者：
Kohler, A;Schwindling, S;Conrath, U
通讯作者：
Conrath, U