Unmyelinated fibers of the anterior ethmoidal nerve in the rat co-localize with neurons in the medullary dorsal horn and ventrolateral medulla activated by nasal stimulation.
Unmyelinated fibers of the anterior ethmoidal nerve in the rat co-localize with neurons in the medullary dorsal horn and ventrolateral medulla activated by nasal stimulation.
复制标题
大鼠中前筛神经的无髓纤维与鼻刺激激活的髓质背角和腹侧髓质中的神经元共定位。
DOI：
10.1016/j.brainres.2009.08.077
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发表时间：
2009-11-17
期刊：
影响因子：
2.9
通讯作者：
McCulloch PF
中科院分区：
文献类型：
--
作者：
Hollandsworth MP;DiNovo KM;McCulloch PF
筛前神经（AEN）支配鼻通道和外鼻孔，并作为鼻咽和潜水反应的传入分支。然而，尽管65%的AEN由无髓纤维组成，但尚未确定这种传入信号是由无髓纤维还是有髓纤维携带。用WGA-HRP、IB_4-HRP和CTB-HRP跨神经节示踪剂追踪大鼠AEN的中枢投射。标记模式的解释表明，AEN无髓纤维项目主要是同侧髓背角（MDH）的腹侧尖端的最后区的水平。其他无髓投射则指向腹侧三叉旁核和延髓腹外侧区，特别是Bötzinger和RVLM/C1区。有髓AEN纤维投射到三叉神经腹侧旁核和三叉神经中脑核。用氨蒸汽刺激麻醉大鼠的鼻道，会产生包括呼吸暂停、心动过缓和动脉血压升高在内的鼻咽反应。AEN的中枢投射与MDH和RVLM/C1内的神经元共定位，这些神经元被鼻刺激激活。腹侧MDH内AEN终末投射的密度与激活神经元的头尾向位置呈正相关，特别是在和只是尾向的obex。我们的结论是，无髓AEN终端的预测参与激活的神经元在MDH和延髓腹外侧参与在大鼠的鼻咽反应。我们还发现，IB 4-HRP是一个比WGA-HRP弱得多的示踪剂。
The anterior ethmoidal nerve (AEN) innervates the nasal passages and external nares, and serves as the afferent limb of the nasopharyngeal and diving responses. However, although 65% of the AEN is composed of unmyelinated fibers, it has not been determined whether this afferent signal is carried by unmyelinated or myelinated fibers. We used the transganglionic tracers WGA-HRP, IB4-HRP, and CTB-HRP to trace the central projections of the AEN of the rat. Interpretation of the labeling patterns suggests that AEN unmyelinated fibers project primarily to the ventral tip of the ipsilateral medullary dorsal horn (MDH) at the level of the area postrema. Other unmyelinated projections were to the ventral paratrigeminal nucleus and ventrolateral medulla, specifically the Bötzinger and RVLM/C1 regions. Myelinated AEN fibers projected to the ventral paratrigeminal and mesencephalic trigeminal nuclei. Stimulating the nasal passages of urethane-anesthetized rats with ammonia vapors produced the nasopharyngeal response that included apnea, bradycardia and an increase in arterial blood pressure. Central projections of the AEN co-localized with neurons within both MDH and RVLM/C1 that were activated by nasal stimulation. Within the ventral MDH the density of AEN terminal projections positively correlated with the rostral-caudal location of activated neurons, especially at and just caudal to the obex. We conclude that unmyelinated AEN terminal projections are involved in the activation of neurons in the MDH and ventrolateral medulla that participate in the nasopharyngeal response in the rat. We also found that IB4-HRP was a much less robust tracer than WGA-HRP.
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DOI：
10.1007/bf01268087
发表时间：
1994-12-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
KITCHENER, PD;LAPIZ, MDS;SNOW, PJ
通讯作者：
SNOW, PJ
影响因子：
3.2
作者：
LIU, HT;LLEWELLYNSMITH, IJ;BASBAUM, AI
通讯作者：
BASBAUM, AI
DOI：
10.1016/0165-1838(91)90077-g
发表时间：
1991-06-01
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
MASUDA, N;TERUI, N;KUMADA, M
通讯作者：
KUMADA, M
影响因子：
3
作者：
ERICSON, H;BLOMQVIST, A
通讯作者：
BLOMQVIST, A
影响因子：
2.9
作者：
McCulloch, PF;Faber, KM;Panneton, WM
通讯作者：
Panneton, WM