Direct isolation in eggs of influenza A (H1N1) and B viruses with haemagglutinins of different antigenic and amino acid composition.
Direct isolation in eggs of influenza A (H1N1) and B viruses with haemagglutinins of different antigenic and amino acid composition.
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使用不同抗原和氨基酸组成的血凝素在鸡蛋中直接分离甲型 (H1N1) 和乙型流感病毒。
DOI：
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发表时间：
1991
影响因子：
3.8
通讯作者：
G. Schild
中科院分区：
文献类型：
--
作者：
J. Oxford;R. Newman;T. Corcoran;J. Bootman;D. Major;P. Yates;J. Robertson;G. Schild
通过经典技术在含胚鸡蛋的羊膜腔中分离来自临床样品的甲型H1N1流感病毒和B流感病毒，并在尿囊腔中增殖。用来自相同临床样品的病毒材料接种来自不同鸡蛋的病毒子代具有抗原性可区分的血凝素（HA）。一些鸡蛋的病毒后代具有HA，这些HA在血清学上与在哺乳动物（MDCK）细胞中平行分离的病毒相同。这些鸡蛋生长的病毒具有HA与哺乳动物细胞生长的病毒的抗原表型似乎是抗原性相关的流行性流感病毒，因为感染后的人血清反应，以高滴度的病毒HA。在直接从鸡蛋中分离的病毒HA中检测到特定的核苷酸变化，对于甲型H1N1流感病毒，在位置163和189处，或者对于B流感病毒，在位置141和196至198处。具有哺乳动物细胞生长病毒的抗原表型的蛋分离的病毒在位置163和196处保留糖基化位点。直接从鸡胚中分离的病毒具有MDCK细胞培养病毒的HA抗原表型和糖基化位点，与后者病毒本身不同，可用作候选流感疫苗病毒。
Influenza A (H1N1) and influenza B viruses from clinical samples were isolated in the amniotic cavity of embryonated hens' eggs by classical techniques and propagated in the allantoic cavity. Virus progeny from different eggs which had been inoculated with virus material from the same clinical sample possessed antigenically distinguishable haemagglutinins (HAs). Virus progeny of some eggs possessed HAs which were serologically identical to those of virus isolated in parallel in mammalian (MDCK) cells. These egg-grown viruses possessing HAs with the antigenic phenotype of mammalian cell-grown viruses appeared to be antigenically related to epidemic influenza virus because post-infection human sera reacted to high titre with the virus HA. Specific nucleotide changes were detected in the HAs of the viruses isolated directly in eggs at positions 163 and 189 for influenza A (H1N1) viruses or positions 141 and 196 to 198 for influenza B viruses. Egg-isolated viruses which possessed the antigenic phenotype of mammalian cell-grown viruses retained glycosylation sites at positions 163 and 196. The viruses isolated directly in embryonated hens' eggs which possessed the HA antigenic phenotype and glycosylation sites of MDCK cell-grown virus can, unlike the latter viruses themselves, be used as candidate influenza vaccine viruses.
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DOI：
10.1073/pnas.79.15.4800
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KRYSTAL, M;ELLIOTT, RM;PALESE, P
通讯作者：
PALESE, P
影响因子：
56.9
作者：
HOLLAND, J;SPINDLER, K;VANDEPOL, S
通讯作者：
VANDEPOL, S
乙型流感病毒现场分离株的抗原和生化分析：流行内和流行间变异的证据。
DOI：
10.1099/0022-1317-64-11-2367
发表时间：
1983
期刊：
The Journal of general virology
影响因子：
--
作者：
Oxford,JS;Abbo,H;Corcoran,T;Webster,RG;Smith,AJ;Grilli,EA;Schild,GC
通讯作者：
Schild,GC
来自不同患者的鸡蛋培养的流感病毒的血凝素存在广泛的异质性。
影响因子：
3.7
作者：
Wang,ML;Katz,JM;Webster,RG
通讯作者：
Webster,RG