An autoregulatory feedback loop involving PAP1 and TAS4 in response to sugars in Arabidopsis.
An autoregulatory feedback loop involving PAP1 and TAS4 in response to sugars in Arabidopsis.
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DOI：
10.1007/s11103-011-9778-9
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发表时间：
2012-09
影响因子：
5.1
通讯作者：
Rock, Christopher D.
中科院分区：
文献类型：
--
作者：
Luo, Qing-Jun;Mittal, Amandeep;Jia, Fan;Rock, Christopher D.
拟南芥中的 miR828 触发反式作用 siRNA 基因 4 (TAS4) 转录物的裂解和小干扰 RNA (ta-siRNA) 的产生。一个 siRNA，TAS4-siRNA81(−)，靶向一组 MYB 转录因子，包括调节花青素生物合成途径的 PAP1、PAP2 和 MYB113。有趣的是，miR828 还靶向 MYB113，这表明这些 MYB、miR828 和 TAS4 之间存在密切关系，但它们的进化起源尚不清楚。我们发现 PAP1、PAP2 和 TAS4 的表达是由幼苗中蔗糖和葡萄糖的外源处理特异性诱导的。脱落酸 (ABA) 途径突变体中的诱导作用减弱，尤其是 PAP1 或 PAP2 的 abi3-1 和 abi5-1 中，而 TAS4 则没有观察到这种效应。 PAP1 受 TAS4 调节，PAP1 转录本和花青素在 ta-siRNA 生物发生途径突变体中的积累证明了这一点。 TAS4-siR81(−) 表达由生理浓度的 Suc 和 Glc 诱导，并且在 pap1-D（激活标记细胞系）中诱导，表明 PAP1 和 TAS4 之间存在反馈调节环。生物信息分析显示，MIR828 同源物存在于双子叶植物和裸子植物中，但仅存在于一种基础单子叶植物中，而 TAS4 只存在于双子叶植物中。与这一观察结果一致，PAP1、PAP2和MYB113双子叶植物旁系同源物显示TAS4-siR81(−)结合位点的肽和核苷酸足迹，为与单子叶植物相比的纯化选择提供了证据。 MIR828、MYB 和 TAS4 之间的扩展序列相似性支持反向复制模型，即 MIR828 从祖先裸子植物 MYB 基因进化而来，并随后通过 miR828* 臂的复制形成 TAS4。我们通过修饰 5'-RACE 获得了树脂松中由 miR828 引导的 MYB mRNA 裂解产物的证据。综上所述，我们的结果表明高等植物中 TAS4 和 miR828 对花青素生物合成的调节具有进化意义，并且与自双子叶植物-单子叶植物分化以来 TAS4 的进化一致。
miR828 in Arabidopsis triggers the cleavage of Trans-Acting SiRNA Gene 4 (TAS4) transcripts and production of small interfering RNAs (ta-siRNAs). One siRNA, TAS4-siRNA81(−), targets a set of MYB transcription factors including PAP1, PAP2, and MYB113 which regulate the anthocyanin biosynthesis pathway. Interestingly, miR828 also targets MYB113, suggesting a close relationship between these MYBs, miR828, and TAS4, but their evolutionary origins are unknown. We found that PAP1, PAP2, and TAS4 expression is induced specifically by exogenous treatment with sucrose and glucose in seedlings. The induction is attenuated in abscisic acid (ABA) pathway mutants, especially in abi3-1 and abi5-1 for PAP1 or PAP2, while no such effect is observed for TAS4. PAP1 is under regulation by TAS4, demonstrated by the accumulation of PAP1 transcripts and anthocyanin in ta-siRNA biogenesis pathway mutants. TAS4-siR81(−) expression is induced by physiological concentrations of Suc and Glc and in pap1-D, an activation-tagged line, indicating a feedback regulatory loop exists between PAP1 and TAS4. Bioinformatic analysis revealed MIR828 homologues in dicots and gymnosperms, but only in one basal monocot, whereas TAS4 is only found in dicots. Consistent with this observation, PAP1, PAP2, and MYB113 dicot paralogs show peptide and nucleotide footprints for the TAS4-siR81(−) binding site, providing evidence for purifying selection in contrast to monocots. Extended sequence similarities between MIR828, MYBs, and TAS4 support an inverted duplication model for the evolution of MIR828 from an ancestral gymnosperm MYB gene and subsequent formation of TAS4 by duplication of the miR828* arm. We obtained evidence by modified 5′-RACE for a MYB mRNA cleavage product guided by miR828 in Pinus resinosa. Taken together, our results suggest that regulation of anthocyanin biosynthesis by TAS4 and miR828 in higher plants is evolutionarily significant and consistent with the evolution of TAS4 since the dicot—monocot divergence.
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