Transferability of Newly Developed Pear SSR Markers to Other Rosaceae Species.
Transferability of Newly Developed Pear SSR Markers to Other Rosaceae Species.
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新开发的梨 SSR 标记向其他蔷薇科物种的可转移性
DOI：
10.1007/s11105-013-0586-z
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发表时间：
2013
影响因子：
2.1
通讯作者：
Wu, J.
中科院分区：
文献类型：
--
作者：
Fan, L.;Zhang, M. -Y.;Liu, Q. -Z.;Li, L. -T.;Song, Y.;Wang, L. -F.;Zhang, S. -L.;Wu, J.
从新组装的梨序列中开发出一组 120 个简单序列重复 (SSR)，并评估来自不同遗传背景的七种梨基因型的多态性。其中，67 (55.8%) 引物对产生多态性扩增。 67 个 SSR 总共检测到 277 个等位基因，平均每个位点有 4.13 个等位基因。对随机挑选的基因座 NAUPy31a 和 NAUpy53a 的扩增产物进行测序，验证了 SSR 基因座的存在。当对蔷薇科 8 个物种的 96 个个体成员测试 67 个引物对时，61.2% (41/67) 的测试 SSR 成功扩增了至少一个蔷薇科属的 PCR 产物。从梨到不同物种的可转移性从 58.2%（苹果）到 11.9%（樱桃）不等。可转移性比率也反映了苹果亚科内部的亲缘关系比李亚科内部的亲缘关系更为密切。两个梨SSR标记NAUpy43c和NAUpy55k可以彻底地区分20种不同的苹果基因型，UPGMA聚类分析将它们分为三组，相似度为0.56。梨SSR的高水平多态性和向蔷薇科物种的良好可转移性表明它们有望应用于梨和其他蔷薇科物种的未来分子筛选、图谱构建和比较基因组研究。
A set of 120 simple sequence repeats (SSRs) was developed from the newly assembled pear sequence and evaluated for polymorphisms in seven genotypes of pear from different genetic backgrounds. Of these, 67 (55.8 %) primer pairs produced polymorphic amplifications. Together, the 67 SSRs detected 277 alleles with an average of 4.13 per locus. Sequencing of the amplification products from randomly picked loci NAUPy31a and NAUpy53a verified the presence of the SSR loci. When the 67 primer pairs were tested on 96 individual members of eight species in the Rosaceae family, 61.2 % (41/67) of the tested SSRs successfully amplified a PCR product in at least one of the Rosaceae genera. The transferability from pear to different species varied from 58.2 % (apple) to 11.9 % (cherry). The ratio of transferability also reflected the closer relationships within Maloideae over Prunoideae. Two pear SSR markers, NAUpy43c and NAUpy55k, could distinguish the 20 different apple genotypes thoroughly, and UPGMA cluster analysis grouped them into three groups at the similarity level of 0.56. The high level of polymorphism and good transferability of pear SSRs to Rosaceae species indicate their promise for application to future molecular screening, map construction, and comparative genomic studies among pears and other Rosaceae species.
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影响因子：
2
作者：
Martinez-Calvo, Jose;Gisbert, Ana Delia;Badenes, Maria L.
通讯作者：
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影响因子：
2.1
作者：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.1
作者：
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通讯作者：
Martinez-Gomez, Pedro