Perfusion preparation of the rat bladder decellularized scaffold.
Perfusion preparation of the rat bladder decellularized scaffold.
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DOI：
10.1016/j.reth.2023.09.005
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发表时间：
2023-12
影响因子：
4.3
通讯作者：
Gao, Fei
中科院分区：
文献类型：
--
作者：
Tan, Jiang;Qiu, Guoping;Wang, Maoqi;Yu, Zhuoyuan;Ling, Xinyi;Aremu, John Ogooluwa;Wang, Chunyu;Liu, Hao;Zhang, Aozhou;Yang, Mei;Gao, Fei
膀胱重建是泌尿外科领域的一个巨大挑战。近年来，灌注方法在组织工程领域取得了可喜的成果。我们采用改进的灌注法制备了膀胱脱细胞支架，可能适用于膀胱重建。采用SDS（0.5%十二烷基硫酸钠）、SDS-SDC（0.5%十二烷基硫酸钠+0.5%脱氧胆酸钠）灌注法制备大鼠膀胱脱细胞支架。通过苏木精-伊红、Masson和DAPI染色评估膀胱脱细胞支架的组织学特征。此外，我们还制备了小鼠膀胱移植模型，以评估支架的再生潜力。苏木精和伊红、Masson和DAPI染色表明SDS-SDC组中几乎没有细胞组分残留。植入1个月后的组织学分析（苏木精-伊红染色、Masson染色）、CD 31和F4/80染色分析显示脱细胞支架具有再生特性，并且SDS-SDC支架具有比SDS支架更好的再生特性。通过灌注法成功制备了大鼠膀胱脱细胞支架。结果表明，SDS-SDC支架具有更好的脱细胞效率和重建能力，为膀胱重建材料的研究提供了新的视角。
Bladder reconstruction is a huge challenge in the field of urology. In recent years, perfusion methods have brought promising results in the field of tissue engineering. We prepared bladder decellularized scaffolds by improved perfusion, which may be suitable for bladder reconstruction. We prepared decellularized scaffolds of rat bladder by perfusion of SDS (0.5% sodium dodecyl sulfate), SDS-SDC (0.5% sodium dodecyl sulfate +0.5% sodium deoxycholate). Histological characteristics of bladder decellularized scaffolds were assessed by Hematoxylin and eosin, Masson, and DAPI staining. Moreover, we also prepared a murine bladder transplantation model to evaluate the regenerative potential of scaffolds. Hematoxylin and eosin, Masson, and DAPI staining indicated almost no cellular component residues in the SDS-SDC group. Histological analysis (hematoxylin and eosin staining, Masson staining), CD31 and F4/80 staining analysis, one month after implantation, revealed that the decellularized scaffolds had regenerative characteristics, and the SDS-SDC scaffold had better regenerative properties than the SDS scaffold. We successfully prepared the decellularized scaffold for the rat bladder by perfusion. Our results showed that the SDS-SDC scaffold had better decellularization efficiency and reconstruction ability than the SDS scaffold, which provides a new perspective on bladder reconstruction materials.
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影响因子：
14
作者：
Brown, AL;Farhat, W;Woodhouse, KA
通讯作者：
Woodhouse, KA
影响因子：
14
作者：
Sullivan, David C.;Mirmalek-Sani, Sayed-Hadi;Yoo, James J.
通讯作者：
Yoo, James J.
DOI：
10.1126/science.1189345
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Petersen TH;Calle EA;Zhao L;Lee EJ;Gui L;Raredon MB;Gavrilov K;Yi T;Zhuang ZW;Breuer C;Herzog E;Niklason LE
通讯作者：
Niklason LE
影响因子：
6.6
作者：
Jayo, Manuel J.;Jain, Deepak;Bertram, Timothy A.
通讯作者：
Bertram, Timothy A.
影响因子：
3.3
作者：
Consolo, F.;Brizzola, S.;Soncini, M.
通讯作者：
Soncini, M.