Augmentation of the insufficient tissue bed for surgical repair of hypospadias using acellular matrix grafts: A proof of concept study.
Augmentation of the insufficient tissue bed for surgical repair of hypospadias using acellular matrix grafts: A proof of concept study.
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使用acellular基质移植物的组织床的不足组织床的外科手术修复：概念研究证明。
DOI：
10.1177/2041731421998840
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发表时间：
2021-01
影响因子：
8.2
通讯作者：
Southgate J
中科院分区：
文献类型：
--
作者：
Morgante D;Radford A;Abbas SK;Ingham E;Subramaniam R;Southgate J
据报道，组织脱细胞产生的脱细胞基质具有组织整合特性。我们研究了在自然组织床不足以支持持久手术修复的情况下，在手术过程中纳入脱细胞基质移植物的可能性。尿道下裂是一种常见的需要手术的先天性缺陷，但由于修复部位宿主组织床的质量和数量缺陷而导致长期并发症。将生物材料作为单个覆盖移植物植入雄性猪的尿道周围位置。比较了两种脱细胞组织基质：全层猪脱细胞膀胱基质 (PABM) 和商业来源的猪真皮交联脱细胞基质 (Permacol™)。术后 3 个月评估解剖学和免疫组织学结果。没有出现并发症，手术部位进行了全面的美容修复。 PABM 移植物已完全融合，而 Permacol™ 移植物仍然可触及。免疫组织化学分析表明对两种生物材料的非炎症性重塑型反应。 PABM 植入物显示出广泛的基质细胞浸润和新血管形成，细胞密度显着高于 Permacol™（p<0.001），而 Permacol™ 的细胞化和部分封装较差。这项研究支持非交联脱细胞基质的抗炎和组织整合性质，并为在手术过程中（例如在原发性复杂尿道下裂修复中）掺入脱细胞基质提供了原理验证。
Acellular matrices produced by tissue decellularisation are reported to have tissue integrative properties. We examined the potential for incorporating acellular matrix grafts during procedures where there is an inadequate natural tissue bed to support an enduring surgical repair. Hypospadias is a common congenital defect requiring surgery, but associated with long-term complications due to deficiencies in the quality and quantity of the host tissue bed at the repair site. Biomaterials were implanted as single on-lay grafts in a peri-urethral position in male pigs. Two acellular tissue matrices were compared: full-thickness porcine acellular bladder matrix (PABM) and commercially-sourced cross-linked acellular matrix from porcine dermis (Permacol™). Anatomical and immunohistological outcomes were assessed 3 months post-surgery. There were no complications and surgical sites underwent full cosmetic repair. PABM grafts were fully incorporated, whilst Permacol™ grafts remained palpable. Immunohistochemical analysis indicated a non-inflammatory, remodelling-type response to both biomaterials. PABM implants showed extensive stromal cell infiltration and neovascularisation, with a significantly higher density of cells (p < 0.001) than Permacol™, which showed poor cellularisation and partial encapsulation. This study supports the anti-inflammatory and tissue-integrative nature of non-crosslinked acellular matrices and provides proof-of-principle for incorporating acellular matrices during surgical procedures, such as in primary complex hypospadias repair.
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影响因子：
2.9
作者：
Greaves, Nicholas S.;Benatar, Brian;Bayat, Ardeshir
通讯作者：
Bayat, Ardeshir
影响因子：
3.7
作者：
Greaves NS;Lqbal SA;Morris J;Benatar B;Alonso-Rasgado T;Baguneid M;Bayat A
通讯作者：
Bayat A
影响因子：
8.2
作者：
Khan, Umair;Bayat, Ardeshir
通讯作者：
Bayat, Ardeshir
影响因子：
2.6
作者：
Abbas TO;Mahdi E;Hasan A;AlAnsari A;Pennisi CP
通讯作者：
Pennisi CP
影响因子：
4.5
作者：
Kimuli, M;Eardley, I;Southgate, J
通讯作者：
Southgate, J