FfVel1 and FfLae1, components of a velvet-like complex in Fusarium fujikuroi, affect differentiation, secondary metabolism and virulence.
FfVel1 and FfLae1, components of a velvet-like complex in Fusarium fujikuroi, affect differentiation, secondary metabolism and virulence.
复制标题
DOI：
10.1111/j.1365-2958.2010.07263.x
复制
发表时间：
2010-08
影响因子：
3.6
通讯作者：
Tudzynski B
中科院分区：
文献类型：
--
作者：
Wiemann P;Brown DW;Kleigrewe K;Bok JW;Keller NP;Humpf HU;Tudzynski B
除了工业生产的赤霉素（GAs），藤仓镰刀菌能够产生额外的次级代谢产物，如色素bikaverin和脉孢黄质以及真菌毒素伏马菌素和镰孢菌素C。对这些生物合成途径的全球调控知之甚少。最近，天鹅绒复合物包含VeA和其他几个调节蛋白被证明参与了构巢曲霉次生代谢和分化的全球调节。在这里，我们报告的两个组成部分的F。fujikuroi天鹅绒样复合物，FfVel1和FfLae1。在Δ Ffvel 1微阵列研究中，编码这种首次报道的散囊菌科以外的LaeA直系同源物的基因被上调，并且FfLae1在细胞核中与FfVel 1相互作用。Ffvel1和Fflae1的缺失首次揭示了鹿茸同时作为次级代谢的正调节因子（GAs、伏马菌素和镰刀菌素C）和负调节因子（bikaverin），并且这两种成分都影响着F.藤仓。此外，绒状蛋白FfVel2显示类似的功能，分生孢子，次生代谢和毒力的FfVel1。天鹅绒复杂的组件突变体之间的镰刀菌属，曲霉属和青霉属的跨属互补研究支持这个复杂的古老起源，经历了一个分歧，在特定的功能介导的发展和次生代谢。
Besides industrially produced gibberellins (GAs), Fusarium fujikuroi is able to produce additional secondary metabolites such as the pigments bikaverin and neurosporaxanthin and the mycotoxins fumonisins and fusarin C. The global regulation of these biosynthetic pathways is only poorly understood. Recently, the velvet complex containing VeA and several other regulatory proteins was shown to be involved in global regulation of secondary metabolism and differentiation in Aspergillus nidulans. Here we report on the characterization of two components of the F. fujikuroi velvet-like complex, FfVel1 and FfLae1. The gene encoding this first reported LaeA ortholog outside the class of Eurotiomycetidae is upregulated in ΔFfvel1 microarray-studies and FfLae1 interacts with FfVel1 in the nucleus. Deletion of Ffvel1 and Fflae1 revealed for the first time that velvet can simultaneously act as positive (GAs, fumonisins and fusarin C) and negative (bikaverin) regulator of secondary metabolism, and that both components affect conidiation and virulence of F. fujikuroi. Furthermore, the velvet-like protein FfVel2 revealed similar functions regarding conidiation, secondary metabolism and virulence as FfVel1. Cross genus complementation studies of velvet complex component mutants between Fusarium, Aspergillus and Penicillium support an ancient origin for this complex which has undergone a divergence in specific functions mediating development and secondary metabolism.
登录
查看更多内容
影响因子：
3
作者：
Brown, DW;Cheung, F;Whitelaw, CA
通讯作者：
Whitelaw, CA
影响因子：
4.4
作者：
Desjardins, AE;Manandhar, HK;Maragos, CM
通讯作者：
Maragos, CM
影响因子：
3.6
作者：
Bok, Jin Woo;Noordermeer, Daan;Keller, Nancy P.
通讯作者：
Keller, Nancy P.
影响因子：
56.9
作者：
Bayram, Oezguer;Krappmann, Sven;Braus, Gerhard H.
通讯作者：
Braus, Gerhard H.
影响因子：
4.4
作者：
Calvo, AM;Bok, J;Keller, NP
通讯作者：
Keller, NP