Global Transcriptome Analysis of Brown Adipose Tissue of Diet-Induced Obese Mice.
Global Transcriptome Analysis of Brown Adipose Tissue of Diet-Induced Obese Mice.
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DOI：
10.3390/ijms19041095
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发表时间：
2018-04-06
影响因子：
5.6
通讯作者：
Shi H
中科院分区：
文献类型：
--
作者：
Cao J;Zhu Q;Liu L;Glazier BJ;Hinkel BC;Liang C;Shi H
高脂肪饮食（HFD）的摄入会促进肥胖的发生，肥胖是一种因能量摄入与能量消耗不平衡而导致的疾病。棕色脂肪组织（BAT）具有燃烧卡路里产生热量的生热能力，是治疗和预防肥胖的潜在目标。关于 HFD 对 BAT 转录组影响的信息有限。我们假设 HFD 引起的肥胖会导致 BAT 基因的转录调控。 RNA 测序用于从低脂饮食 (LFD) 喂养的瘦小鼠和 HFD 喂养的肥胖小鼠的 BAT 中生成全局转录组图谱。基因本体论 (GO) 分析发现，与免疫反应相关的生物过程 (BP) 相关的基因表达增加，从而增强了趋化因子活性中的分子功能 (MF)；与离子转运和肌肉结构发育相关的 BP 相关基因的表达减少，从而降低了 MF 通道和转运蛋白的活性以及结构结合。京都基因和基因组百科全书 (KEGG) 功能通路分析表明，HFD 增强了与先天免疫相关的通路，而 HFD 抑制了与肌肉收缩和钙信号传导相关的通路。总的来说，这些结果表明饮食引起的肥胖改变了 BAT 的转录特征，导致炎症、钙信号传导、离子运输和细胞结构发育等功能障碍。
Consumption of a high-fat diet (HFD) promotes the development of obesity, a disease resulting from an imbalance between energy intake and energy expenditure. Brown adipose tissue (BAT) has thermogenic capacity that burns calories to produce heat, and it is a potential target for the treatment and prevention of obesity. There is limited information regarding the impact of HFD on the BAT transcriptome. We hypothesized that HFD-induced obesity would lead to transcriptional regulation of BAT genes. RNA sequencing was used to generate global transcriptome profiles from BAT of lean mice fed with a low-fat diet (LFD) and obese mice fed with a HFD. Gene Ontology (GO) analysis identified increased expression of genes involved in biological processes (BP) related to immune responses, which enhanced molecular function (MF) in chemokine activity; decreased expression of genes involved in BP related to ion transport and muscle structure development, which reduced MF in channel and transporter activity and structural binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional pathway analysis indicated that pathways associated with innate immunity were enhanced by HFD, while pathways associated with muscle contraction and calcium signaling were suppressed by HFD. Collectively, these results suggest that diet-induced obesity changes transcriptomic signatures of BAT, leading to dysfunction involving inflammation, calcium signaling, ion transport, and cell structural development.
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DOI：
10.4049/jimmunol.1000900
发表时间：
2010-08-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ellis SL;Gysbers V;Manders PM;Li W;Hofer MJ;Müller M;Campbell IL
通讯作者：
Campbell IL
DOI：
10.1097/med.0b013e328337a81f
发表时间：
2010-04
期刊：
Current opinion in endocrinology, diabetes, and obesity
影响因子：
--
作者：
Cypess AM;Kahn CR
通讯作者：
Kahn CR
影响因子：
4.4
作者：
Contreras C;Gonzalez F;Fernø J;Diéguez C;Rahmouni K;Nogueiras R;López M
通讯作者：
López M
影响因子：
2.5
作者：
Cui X;Nguyen NL;Zarebidaki E;Cao Q;Li F;Zha L;Bartness T;Shi H;Xue B
通讯作者：
Xue B
影响因子：
3.5
作者：
Daikoku, T;Shinohara, Y;Terada, H
通讯作者：
Terada, H