Osteoblasts responses to three-dimensional nanofibrous gelatin scaffolds.
Osteoblasts responses to three-dimensional nanofibrous gelatin scaffolds.
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DOI：
10.1002/jbm.a.34253
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发表时间：
2012-11
影响因子：
4.9
通讯作者：
Liu, Xiaohua
中科院分区：
文献类型：
--
作者：
Sachar, Ashneet;Strom, T. Amanda;Serrano, Maria J.;Benson, M. Douglas;Opperman, Lynne A.;Svoboda, Kathy K. H.;Liu, Xiaohua
开发适合骨组织工程的支架需要深入了解成骨细胞和支架生物材料之间的相互作用。尽管已经积累了大量关于二维 (2D) 平面基底上细胞与材料相互作用的知识，但我们对成骨细胞如何响应仿生纳米结构三维 (3D) 支架的理解非常有限。在这项工作中，我们开发了一种方法，使用共聚焦显微镜作为可视化、分析和量化 3D 纳米纤维明胶支架 (3D-NF-GS) 上成骨细胞基质相互作用和骨组织形成的有效工具。整合素 β1、磷桩蛋白和纽蛋白用于检测成骨细胞对 3D-NF-GS 纳米纤维结构的反应。与在 2D 基质上培养的成骨细胞不同，接种在 3D-NF-GS 上的成骨细胞显示出较少的磷酸桩蛋白和纽蛋白粘着斑，并且在前 5 天后很难检测到整合素 β1。 3D-NF-GS 上的骨唾液蛋白 (BSP) 表达主要存在于第 5 天的细胞质中和第 2 周的分泌囊泡内部，而 2D 明胶基质上的大部分 BSP 集中在朝向外围的细胞界面或粘着斑位点。共焦图像显示，成骨细胞能够在 5 天内在整个 3D 矩阵中迁移。 14 天时，成骨细胞在支架孔内组织为结节状聚集体，大量胶原蛋白和其他细胞分泌物覆盖并重塑了 3D-NF-GS 的表面。培养2周后，这些结节矿化并均匀分布在整个3D-NF-GS内部。总而言之，这些结果让我们深入了解仿生纳米纤维 3D 支架中的成骨细胞与基质的相互作用，并将指导骨组织工程最佳支架的开发。
The development of suitable scaffolds for bone tissue engineering requires an in-depth understanding of the interactions between osteoblasts and scaffolding biomaterials. Although there have been a large amount of knowledge accumulated on the cell-material interactions on two-dimensional (2D) planar substrates, our understanding of how osteoblasts respond to a biomimetic nano-structured three-dimensional (3D) scaffold is very limited. In this work, we developed an approach to use confocal microscopy as an effective tool for visualizing, analyzing and quantifying osteoblast-matrix interactions and bone tissue formation on 3D nanofibrous gelatin scaffolds (3D-NF-GS). Integrin β1, phosphor-paxillin, and vinculin were used to detect osteoblasts responses to the nanofibrous architecture of 3D-NF-GS. Unlike osteoblasts cultured on 2D substrates, osteoblasts seeded on 3D-NF-GS showed less focal adhesions for phospho-paxillin and vinculin and the integrin β1 was difficult to detect after the first 5 days. Bone sialoprotein (BSP) expression on the 3D-NF-GS was present mainly in the cell cytoplasm at 5 days and inside secretory vesicles at 2 weeks, whereas most of the BSP on the 2D gelatin substrates was concentrated either in cell interface towards the periphery or at focal adhesion sites. Confocal images showed that osteoblasts were able to migrate throughout the 3D matrix within 5 days. By 14 days, osteoblasts were organized as nodular aggregations inside the scaffold pores and a large amount of collagen and other cell secretions covered and remodeled the surfaces of the 3D-NF-GS. These nodules were mineralized and were uniformly distributed inside the entire 3D-NF-GS after being cultured for 2 weeks. Taken together, these results give insight into osteoblast-matrix interactions in biomimetic nanofibrous 3D scaffolds and will guide the development of optimal scaffolds for bone tissue engineering.
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用于骨组织工程的仿生纳米纤维明胶/磷灰石复合支架。
影响因子：
14
作者：
Liu, Xiaohua;Smith, Laura A.;Hu, Jiang;Ma, Peter X.
通讯作者：
Ma, Peter X.
影响因子：
4.9
作者：
Liu, XH;Won, YJ;Ma, PX
通讯作者：
Ma, PX
影响因子：
2.9
作者：
Liu, Xiaohua;Smith, Laura;Ma, Peter X.
通讯作者：
Ma, Peter X.
DOI：
10.1083/jcb.96.3.639
发表时间：
1983-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ecarot-Charrier B;Glorieux FH;van der Rest M;Pereira G
通讯作者：
Pereira G
影响因子：
3.2
作者：
BIANCO, P;RIMINUCCI, M;ROBEY, PG
通讯作者：
ROBEY, PG