Effects of resveratrol supplementation on bone growth in young rats and microarchitecture and remodeling in ageing rats.
Effects of resveratrol supplementation on bone growth in young rats and microarchitecture and remodeling in ageing rats.
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DOI：
10.3390/nu6125871
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发表时间：
2014-12-16
期刊：
影响因子：
5.9
通讯作者：
Xian CJ
中科院分区：
文献类型：
--
作者：
Lee AM;Shandala T;Nguyen L;Muhlhausler BS;Chen KM;Howe PR;Xian CJ
骨质疏松症是一种在老年人中非常普遍的骨骼疾病，会导致严重的骨折。在青春期达到的峰值骨量已被证明可以预测以后的生活中骨量和骨质疏松相关的骨折风险。白藜芦醇是一种天然多酚化合物，可能具有促进骨形成和减少骨吸收的潜力。然而，目前尚不清楚它是否能在快速生长过程中帮助骨骼生长和骨量积累，以及在衰老过程中调节骨骼代谢。利用大鼠模型，本研究调查了在出生后快速生长期和成年后期（早期衰老）补充白藜芦醇对骨微结构和代谢的潜在影响。在生长试验中，4周龄雄性带帽Wistar大鼠正常饮食，给予白藜芦醇（2.5 mg/kg/d）或对照，持续5周。在衰老试验中，6月龄雄性带帽Wistar大鼠给予白藜芦醇（20mg /kg/天）或载药治疗3个月。采用μ-计算机断层扫描（μ-CT）、骨组织形态学测量和逆转录-聚合酶链反应（RT-PCR）基因表达分析检测治疗对胫骨的影响。在青年动物模型中，白藜芦醇处理对骨小梁体积和骨重塑指标没有影响。早期衰老大鼠补充白藜芦醇有降低骨小梁体积、Sirt1基因表达和增加骨中脂肪生成相关基因表达的趋势，但均无统计学意义。然而，骨钙素表达降低（p = 0.03）。此外，白藜芦醇补充组血清骨吸收标志物c末端末端肽I型胶原蛋白（CTX-1）水平显著升高（p = 0.02），血清骨形成标志物碱性磷酸酶（ALP）水平未见变化。这些大鼠模型的结果表明，在快速生长阶段，补充白藜芦醇不会显著影响骨体积，但可能对早期衰老的男性骨骼产生潜在的负面影响。
Osteoporosis is a highly prevalent skeletal disorder in the elderly that causes serious bone fractures. Peak bone mass achieved at adolescence has been shown to predict bone mass and osteoporosis related risk fracture later in life. Resveratrol, a natural polyphenol compound, may have the potential to promote bone formation and reduce bone resorption. However, it is unclear whether it can aid bone growth and bone mass accumulation during rapid growth and modulate bone metabolism during ageing. Using rat models, the current study investigated the potential effects of resveratrol supplementation during the rapid postnatal growth period and in late adulthood (early ageing) on bone microarchitecture and metabolism. In the growth trial, 4-week-old male hooded Wistar rats on a normal chow diet were given resveratrol (2.5 mg/kg/day) or vehicle control for 5 weeks. In the ageing trial, 6-month-old male hooded Wistar rats were treated with resveratrol (20 mg/kg/day) or vehicle for 3 months. Treatment effects in the tibia were examined by μ-computer tomography (μ-CT) analysis, bone histomorphometric measurements and reverse transcription-polymerase chain reaction (RT-PCR) gene expression analysis. Resveratrol treatment did not affect trabecular bone volume and bone remodeling indices in the youth animal model. Resveratrol supplementation in the early ageing rats tended to decrease trabecular bone volume, Sirt1 gene expression and increased expression of adipogenesis-related genes in bone, all of which were statistically insignificant. However, it decreased osteocalcin expression (p = 0.03). Furthermore, serum levels of bone resorption marker C-terminal telopeptides type I collagen (CTX-1) were significantly elevated in the resveratrol supplementation group (p = 0.02) with no changes observed in serum levels of bone formation marker alkaline phosphatase (ALP). These results in rat models suggest that resveratrol supplementation does not significantly affect bone volume during the rapid growth phase but may potentially have negative effects on male skeleton during early ageing.
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影响因子：
7.1
作者：
Fischer-Posovszky, Pamela;Kukulus, Vera;Wabitsch, Martin
通讯作者：
Wabitsch, Martin
影响因子：
4.2
作者：
Draper, CR;Edel, MJ;Prince, RL
通讯作者：
Prince, RL
DOI：
10.1093/gerona/glt046
发表时间：
2013-10-01
影响因子：
5.1
作者：
Jilka, Robert L.
通讯作者：
Jilka, Robert L.
影响因子：
7.4
作者：
Juan, ME;Lamuela-Raventós, RM;Planas, JM
通讯作者：
Planas, JM
影响因子：
3.3
作者：
Habold, Caroline;Momken, Iman;Brasse, David
通讯作者：
Brasse, David