Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity.
Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity.
复制标题
DOI：
10.1371/journal.pone.0087661
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Shimomura I
中科院分区：
文献类型：
--
作者：
Inoue K;Maeda N;Mori T;Sekimoto R;Tsushima Y;Matsuda K;Yamaoka M;Suganami T;Nishizawa H;Ogawa Y;Funahashi T;Shimomura I
肥胖是一种流行病，增加了心血管疾病和代谢紊乱如2型糖尿病的风险。我们最近研究了内脏脂肪肥胖和人类受试者外周血细胞基因表达谱之间的关联。在一系列的研究中，Opa（淋病奈瑟菌不透明相关）相互作用蛋白5（OIP 5）被提名为脂肪细胞中功能未知的分子，因此本研究旨在研究OIP 5在肥胖中的作用。产生过表达Oip 5的腺病毒（Ad-Oip 5），并感染稳定表达柯萨奇-腺病毒受体（CAR-3 T3-L1）的3 T3-L1细胞和小鼠皮下脂肪。对于敲减实验，将针对Oip 5的siRNA（Oip 5-siRNA）引入3 T3-L1细胞中。通过BrdU摄取、EdU染色和细胞计数来测量脂肪细胞的增殖。在肥胖的白色脂肪组织中观察到Oip 5 mRNA水平的显著增加，并且在成熟脂肪细胞组分和基质血管细胞组分中都检测到这种增加。Ad-Oip 5感染的CAR-3 T3-L1前脂肪细胞和脂肪细胞迅速增殖，而在Oip 5-siRNA引入的3 T3-L1前脂肪细胞中观察到增殖显著降低。在Ad-Oip 5给药的脂肪组织中，脂肪重量和脂肪细胞数量显著增加。Oip 5促进前脂肪细胞和成熟脂肪细胞的增殖并促进脂肪增生。Oip 5的升高可能与肥胖的发生有关。
Obesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human subjects. In a series of studies, Opa (Neisseria gonorrhoeae opacity-associated)-interacting protein 5 (OIP5) was nominated as a molecule of unknown function in adipocytes and thus the present study was performed to investigate the role of OIP5 in obesity. Adenovirus overexpressing Oip5 (Ad-Oip5) was generated and infected to 3T3-L1 cells stably expressing Coxsackie-Adenovirus Receptor (CAR-3T3-L1) and to mouse subcutaneous fat. For a knockdown experiment, siRNA against Oip5 (Oip5-siRNA) was introduced into 3T3-L1 cells. Proliferation of adipose cells was measured by BrdU uptake, EdU-staining, and cell count. Significant increase of Oip5 mRNA level was observed in obese white adipose tissues and such increase was detected in both mature adipocytes fraction and stromal vascular cell fraction. Ad-Oip5-infected CAR-3T3-L1 preadipocytes and adipocytes proliferated rapidly, while a significant reduction of proliferation was observed in Oip5-siRNA-introduced 3T3-L1 preadipocytes. Fat weight and number of adipocytes were significantly increased in Ad-Oip5-administered fat tissues. Oip5 promotes proliferation of pre- and mature-adipocytes and contributes adipose hyperplasia. Increase of Oip5 may associate with development of obesity.
登录
查看更多内容
影响因子：
64.5
作者：
Rodeheffer, Matthew S.;Birsoy, Kivanc;Friedman, Jeffrey M.
通讯作者：
Friedman, Jeffrey M.
影响因子：
3.7
作者：
Nakamura, Yoshito;Tanaka, Fumiaki;Mori, Masaki
通讯作者：
Mori, Masaki
影响因子：
--
作者：
HIMMSHAGEN, J;CUI, JY;CLAUS, TH
通讯作者：
CLAUS, TH
影响因子：
3.5
作者：
Maeda, K;Okubo, K;Matsubara, K
通讯作者：
Matsubara, K
影响因子：
4.8
作者：
Sakai, Tamon;Sakaue, Hiroshi;Kasuga, Masato
通讯作者：
Kasuga, Masato