Frequency Alterations in Key Innate Immune Cell Components in the Peripheral Blood of Dengue Patients Detected by FACS Analysis
Frequency Alterations in Key Innate Immune Cell Components in the Peripheral Blood of Dengue Patients Detected by FACS Analysis
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FACS 分析检测登革热患者外周血中关键先天免疫细胞成分的频率变化
作者：
J. Tsai;Y. Jen;Jung;Hui;S. Noisakran;G. Perng
登革热是一种计时疾病，登革热患者通常直到出现前驱症状后2-3天才寻求帮助。因此，登革热患者的先天免疫参数在很大程度上仍未被探索。对登革热患者外周血中的关键天然免疫细胞进行了全面的FACS分析。纳入了27名登革热患者，根据血清学结果，他们中的大多数都是原发感染。用流式细胞仪检测NK细胞、血小板-白细胞聚集物(PLA)、炎性单核细胞、浆细胞样细胞(PDC)和髓系树突状细胞(MDC)。FACS结果显示：(1)NK细胞在发热后第5天显著下降，发病后2周内逐渐恢复正常；(2)出现双相的聚乳酸，在第6~8天和11~16天达到最大值，以血小板-单核细胞聚集物(PMA)最常见；(3)炎性单核细胞在第5~8天持续下降，第2周逐渐恢复正常；(4)PDC在第5天达到最大值，1周后逐渐下降至基线水平。相比之下，MDC在患病的第一周有所波动，并在患病的第二周恢复到基线水平。最有趣的发现是两相的解放军，特别是PMA。这些先天免疫学参数可能是决定登革热复杂发病机制的关键因素。
Dengue is a timing disease, and frequently dengue patients do not seek help until 2–3 days after prodrome. Thus, the innate immune parameters in dengue patients remain largely unexplored. A comprehensive FACS profiling of key innate immune cells in the peripheral blood of dengue patients was performed. Twenty-seven dengue patients varying in days of fever onset were enrolled and the majority of them had primary infection based on serological results. FACS panels were used to evaluate NK cells, platelet-leukocyte aggregates (PLA), inflammatory monocytes, and plasmacytoid (pDC) and myeloid dendritic cells (mDC). FACS results showed the following findings: (i) NK cells significantly dropped on day 5 after the onset of fever and gradually returned to normal within 2 weeks of illness; (ii) biphasic PLA were observed, maximum levels were reached on days 6–8 and 11–16, and platelet-monocyte aggregates (PMA) were the most frequent event; (iii) inflammatory monocytes were consistently lower on days 5–8 and gradually returned to the normal level in the second week of illness, and (iv) pDC reached the maximum level on day 5 and gradually declined to the baseline level after 1 week of illness. In contrast, mDC fluctuated somewhat during the first week of illness and returned to the baseline level in the second week of illness. The most interesting finding was the biphasic PLA, in particular the PMA. These innate immunological parameters could be a crucial factor dictating the complicated pathogenesis of dengue disease.
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DOI：
10.4269/ajtmh.1993.48.222
发表时间：
1993-02-01
影响因子：
3.3
作者：
KURANE, I;INNIS, BL;ENNIS, FA
通讯作者：
ENNIS, FA
影响因子：
20.3
作者：
Onlamoon, Nattawat;Noisakran, Sansanee;Perng, Guey Chuen
通讯作者：
Perng, Guey Chuen
影响因子：
6.4
作者：
Vaughn, DW;Green, S;Nisalak, A
通讯作者：
Nisalak, A
影响因子：
3.7
作者：
Diamond, MS;Harris, E
通讯作者：
Harris, E
影响因子：
6.4
作者：
Green, S;Pichyangkul, S;Ennis, FA
通讯作者：
Ennis, FA