Volatile organic compounds of Metarhizium brunneum influence the efficacy of entomopathogenic nematodes in insect control.
Volatile organic compounds of Metarhizium brunneum influence the efficacy of entomopathogenic nematodes in insect control.
复制标题
DOI：
10.1016/j.biocontrol.2020.104527
复制
发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Butt T
中科院分区：
文献类型：
--
作者：
Hummadi EH;Dearden A;Generalovic T;Clunie B;Harrott A;Cetin Y;Demirbek M;Khoja S;Eastwood D;Dudley E;Hazir S;Touray M;Ulug D;Hazal Gulsen S;Cimen H;Butt T
绿僵菌VOCs，1-辛烯-3-醇和3-辛酮，影响EPN的行为。高剂量的1-辛烯-3-醇和3-辛酮可驱除或杀死EPN感染性幼鱼。绿僵菌挥发物降低昆虫宿主的EPN感染性。绿僵菌挥发性有机化合物使昆虫不能移动或杀死昆虫。挥发物影响EPN在宿主体内的繁殖。昆虫病原真菌褐绿僵菌（Metarhiziumbrunneum）与昆虫病原线虫（EPN）具有相同的生态位，两者都在根际竞争昆虫作为食物来源。这些生物防治剂之间的相互作用可以是拮抗的或协同的。为了更好地理解这些相互作用，本研究着重研究了M。棕挥发性有机化合物（VOCs），1-辛烯-3-醇和3-辛酮，对EPN的生存和行为。这些挥发性有机化合物被证明是高毒性的感染性青少年（IJS）的EPN斯氏线虫carpocapsae，斯氏线虫feltiae和异小杆线虫bacteriophora与死亡率呈剂量依赖性。对H.在Pluronic F127凝胶中的细菌IJs显示出与所有测试浓度的对照相比对VOC的显著偏好。3-辛酮对大蜡螟、小菜蛾和象甲幼虫的毒性大于1-辛烯-3-醇。C的死亡率。splendana和G.当暴露于相对高剂量（>25%）的3-辛酮时，大蜡螟幼虫显著更高。较低剂量的3-辛酮和1-辛烯-3-醇使测试昆虫固定，其在暴露于新鲜空气2小时后恢复。对H.细菌属的研究表明，将IJs暴露于> 10%浓度的3-辛酮或1-辛烯-3-醇会对感染性产生负面影响，而暴露于较低剂量（0.1%，0.01%）则没有影响。挥发性有机化合物影响了IJ，降低了穿透效率和G内的世代数。但它们未能抑制细菌共生体Photorhabdus kayaii。挥发性有机化合物的生态意义，以及它们如何影响EPF-EPN昆虫相互作用进行了讨论。
Metarhizium VOCs, 1-octen-3-ol and 3 octanone, influence behaviour of EPN. High doses of 1-octen-3-ol and 3 octanone repel or kill EPN infective juveniles. Metarhizium volatiles reduce EPN infectivity of insect hosts. Metarhizium VOCs immobilise or kill insects. Volatiles affect reproduction of EPN inside host. The entomopathogenic fungus (EPF) Metarhizium brunneum occupies the same ecological niche as entomopathogenic nematodes (EPN), with both competing for insects as a food source in the rhizosphere. Interactions between these biocontrol agents can be antagonistic or synergistic. To better understand these interactions, this study focussed on investigating the effect of M. brunneum volatile organic compounds (VOCs), 1-octen-3-ol and 3-octanone, on EPN survival and behaviour. These VOCs proved to be highly toxic to the infective juveniles (IJs) of the EPN Steinernema carpocapsae, Steinernema feltiae and Heterorhabditis bacteriophora with mortality being dose dependent. Chemotaxis studies of H. bacteriophora IJs in Pluronic F127 gel revealed significant preference for the VOCs compared with controls for all tested concentrations. The VOCs also impacted on the test insects in a dose-dependent manner with 3-octanone being more toxic to Galleria mellonella, Cydia splendana and Curculio elephas larvae than 1-octen-3-ol. Mortality of C. splendana and G. mellonella larvae was significantly higher when exposed to relatively high doses (>25%) of 3-octanone. Lower doses of 3-octanone and 1-octen-3-ol immobilised test insects, which recovered after exposure to fresh air for 2 hrs. In depth studies on H. bacteriophora showed that exposure of IJs to > 10% concentration of 3-octanone or 1-octen-3-ol negatively affected infectivity whereas exposure to lower doses (0.1%, 0.01%) had no effect. The VOCs affected IJs, reducing penetration efficacy and the number of generations inside G. mellonella but they failed to inhibit the bacterial symbiont, Photorhabdus kayaii. The ecological significance of VOCs and how they could influence EPF-EPN insect interactions is discussed.
登录
查看更多内容
影响因子：
9.7
作者：
Gu, Ying-Qi;Mo, Ming-He;Zhang, Ke-Qin
通讯作者：
Zhang, Ke-Qin
影响因子：
--
作者：
Fialho, Mauricio Batista;Bessi, Rosana;Pascholati, Sérgio Florentino
通讯作者：
Pascholati, Sérgio Florentino
影响因子：
4.2
作者：
de Faria, Marcos R.;Wraight, Stephen P.
通讯作者：
Wraight, Stephen P.
anatoliense Steinernema（Rhabditida: Steinernematidae）的生态学特征
影响因子：
3.4
作者：
Gungor, Didern Simsek;Keskin, Nevin;Hazir, Selcuk
通讯作者：
Hazir, Selcuk
影响因子：
2.3
作者：
Davis, Thomas Seth;Landolt, Peter J.
通讯作者：
Landolt, Peter J.