Stabilization of costal cartilage graft warping using infrared laser irradiation in a porcine model.
Stabilization of costal cartilage graft warping using infrared laser irradiation in a porcine model.
复制标题
在猪模型中使用红外激光照射稳定肋软骨移植物的翘曲。
DOI：
10.1001/archfacial.2010.93
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发表时间：
2010-11
影响因子：
--
通讯作者：
Wong, Brian
中科院分区：
文献类型：
--
作者：
Foulad, Allen;Ghasri, Pedram;Garg, Rohit;Wong, Brian
建立一种利用红外激光照射快速稳定猪肋骨软骨外周切片形态变化过程的方法。取40例猪肋骨软骨标本(40×10×2 mm)。其中30个样本立即用λ=1.32μm的激光(光斑大小，2 mm直径)照射，采用3种曝光处理中的一种：6W，2秒，4点；8W，3秒，4点；或6W，2秒，8点。10个对照标本仅浸泡在0.9%生理盐水中。从0分钟、照射后即刻、30分钟、1小时、5小时和24小时拍摄的照片中测量曲率角。红外成像用于测量辐射过程中的表面温度。用活/死实验结合荧光共聚焦显微镜测定照射后的细胞存活率。与未经处理的对照组相比，照射后的移植物在前30分钟内加速形状变化，达到稳定的几何形状。此后，照射后移植物的形状变化很小或没有变化，而对照组在30分钟到24小时的时间内曲度有显著变化(P<；.001)。平均峰值辐照光斑温度范围为76°C至82°C。激光光斑部位的细胞活力测量显示，死亡细胞呈半球形，深度为0.8~1.2 mm，表面直径为1.9~2.7 mm。激光照射外周肋软骨切片，通过加速翘曲过程，为快速稳定急性形态改变提供了一种有效的方法。实现这一目标所需的温度升高在空间上是有限的，并且完全在可容忍的组织损伤的范围内。
To develop a method to rapidly stabilize the shape change process in peripheral slices of costal cartilage by using infrared laser irradiation in a porcine model. Forty peripheral porcine costal cartilage specimens (40×10×2 mm) were harvested. Thirty of these specimens were immediately irradiated with an Nd: YAG laser (λ=1.32 μm; spot size, 2-mm diameter) using 1 of 3 exposure treatments: 6W, 2 seconds, and 4 spots; 8 W, 3 seconds, and 4 spots; or 6 W, 2 seconds, and 8 spots. Ten control specimens were only immersed in 0.9% saline solution. Angle of curvature was measured from photographs taken at 0 minutes, immediately after irradiation, and at 30 minutes, 1 hour, 5 hours, and 24 hours. Infrared imaging was used to measure surface temperatures during irradiation. Cell viability after irradiation was determined using a live/dead assay in conjunction with fluorescent confocal microscopy. Compared with the untreated controls, the irradiated grafts underwent accelerated shape change within the first 30 minutes to reach a stable geometry. Thereafter, irradiated grafts underwent little or no shape change, whereas the control group exhibited significant change in curvature from 30 minutes to 24 hours (P<.001). The average peak irradiated spot temperatures ranged from 76°C to 82°C. Cell viability measurements at the laser spot sites demonstrated a hemispherically shaped region of dead cells with a depth of 0.8 to 1.2 mm and a surface diameter of 1.9 to 2.7 mm. Laser irradiation of peripheral costal cartilage slices provides an effective method for rapidly stabilizing acute shape change by accelerating the warping process. The temperature elevations necessary to achieve this are spatially limited and well within the limits of tolerable tissue injury.
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影响因子：
7.3
作者：
Grogan, SP;Aklin, B;Mainil-Varlet, P
通讯作者：
Mainil-Varlet, P
影响因子：
--
作者：
Lopez, Manuel A.;Shah, Anil R.;Toriumi, Dean M.
通讯作者：
Toriumi, Dean M.
影响因子：
105.7
作者：
MATTHEWS, JNS;ALTMAN, DG;ROYSTON, P
通讯作者：
ROYSTON, P
影响因子：
2.4
作者：
Bagratashvili, VN;Sobol, EN;Howdle, SM
通讯作者：
Howdle, SM
影响因子：
3.5
作者：
Díaz, SH;Nelson, JS;Wong, BJF
通讯作者：
Wong, BJF