3D Bioprinting of Oxygenated Cell-Laden Gelatin Methacryloyl Constructs.
3D Bioprinting of Oxygenated Cell-Laden Gelatin Methacryloyl Constructs.
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DOI：
10.1002/adhm.201901794
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发表时间：
2020-08
影响因子：
10
通讯作者：
Ashammakhi N
中科院分区：
文献类型：
--
作者：
Erdem A;Darabi MA;Nasiri R;Sangabathuni S;Ertas YN;Alem H;Hosseini V;Shamloo A;Nasr AS;Ahadian S;Dokmeci MR;Khademhosseini A;Ashammakhi N
在移植的早期阶段和新血管形成之前的细胞存活是三维生物打印组织翻译应用中的主要挑战。通过过氧化钙(CPO)等氧气产生源向移植细胞补充氧气(O2)是确保细胞活力的一种有吸引力的方法。过氧化钙还会产生氢氧化钙，从而降低生物油墨的粘度，这是生物打印的限制因素。因此，将该解决方案应用于3D生物打印具有重大挑战。在这项研究中，我们开发了一种明胶甲基丙烯酰基(GelMA)生物墨水，并对其pH值和粘度进行了优化。流变性的改善导致了一种坚固的生物墨水的生产，适合3D生物打印和受控的O2释放。此外，我们还对不同CPO浓度的水凝胶的氧气释放、生物打印条件和力学性能进行了表征。作为概念研究的证明，使用含有CPO的GelMA生物墨水对成纤维细胞和心肌细胞进行生物打印。在低氧条件下培养7天后，检测印迹细胞的活性和代谢活性。结果表明，在低氧条件下，CPO的加入提高了生物印迹构建体中细胞的代谢活性和活力。
Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of three-dimensional (3D) bioprinted tissues. Supplementing oxygen (O2) to transplanted cells via an O2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide which reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant challenge. In this study, we developed a gelatin methacryloyl (GelMA) bioink that was optimized in terms of pH and viscosity. The improved rheological properties led to the production of a robust bioink suitable for 3D bioprinting and controlled O2 release. In addition, we characterized O2 release, bioprinting conditions and mechanical performance of hydrogels having different CPO concentrations. As a proof of concept study, fibroblasts and cardiomyocytes were bioprinted using GelMA bioink containing CPO. Viability and metabolic activity of printed cells were checked after seven days of culture under hypoxic condition. The results showed that the addition of CPO improves the metabolic activity and viability of cells in bioprinted constructs under hypoxic condition.
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影响因子：
9
作者：
Hwang CM;Sant S;Masaeli M;Kachouie NN;Zamanian B;Lee SH;Khademhosseini A
通讯作者：
Khademhosseini A
DOI：
10.1016/b978-0-12-394309-5.00006-7
发表时间：
2012
影响因子：
--
作者：
Kalogeris, Theodore;Baines, Christopher P.;Krenz, Maike;Korthuis, Ronald J.
通讯作者：
Korthuis, Ronald J.
影响因子：
5.8
作者：
Abdi, Syed Izhar Haider;Ng, Sing Muk;Lim, Jeong Ok
通讯作者：
Lim, Jeong Ok
影响因子：
4.1
作者：
Chang, Chen-Jung
通讯作者：
Chang, Chen-Jung
影响因子：
41.2
作者：
Montgomery, Miles;Ahadian, Samad;Radisic, Milica
通讯作者：
Radisic, Milica