The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury.
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury.
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DOI：
10.1186/1471-2202-9-97
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发表时间：
2008-10-03
期刊：
影响因子：
2.4
通讯作者：
Schiöth HB
中科院分区：
文献类型：
--
作者：
Pickering C;Hägglund M;Szmydynger-Chodobska J;Marques F;Palha JA;Waller L;Chodobski A;Fredriksson R;Lagerström MC;Schiöth HB
GPR 125属于粘附G蛋白偶联受体（GPCR）家族。在许多脊椎动物基因组中发现了GPR 125的单拷贝。我们还鉴定了果蝇序列DmCG 15744，其与整个组III粘附GPCR共享共同祖先，并且还包含在哺乳动物GPR 125中观察到的IG、LRR和HBD结构域。我们发现GPR 125在脉络丛细胞中的特异性表达，使用原位杂交和蛋白质特异性抗体，并结合原位/免疫组织化学共定位使用细胞角蛋白，上皮细胞特异性标记。LPS诱导的炎症并不改变GPR 125的表达。然而，GPR 125表达在创伤性脑损伤（TBI）后4小时瞬时增加（几乎2倍），随后在脉络丛中从2天起减少（大约4倍），以及在海马中增加表达（2倍），其延迟至损伤后1天。这些发现表明GPR 125在脉络膜和海马对损伤的反应中起功能性作用。
GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125. We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased (almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease (approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression (2-fold) in the hippocampus that was delayed until 1 day after injury. These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
4.7
作者：
Lagerstrom, Malin C.;Rabe, Nadine;Schioth, Helgi B.
通讯作者：
Schioth, Helgi B.
影响因子：
64.8
作者：
Park, Daeho;Tosello-Trampont, Annie-Carole;Ravichandran, Kodi S.
通讯作者：
Ravichandran, Kodi S.
影响因子：
3.4
作者：
Nordström KJ;Fredriksson R;Schiöth HB
通讯作者：
Schiöth HB
影响因子：
2.9
作者：
Endo, H;Sasaki, K;Kayama, T
通讯作者：
Kayama, T