Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues.
Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues.
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DOI：
10.3389/fmicb.2018.02266
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发表时间：
2018
影响因子：
5.2
通讯作者：
Duarte Dos Santos CN
中科院分区：
文献类型：
--
作者：
de Noronha L;Zanluca C;Burger M;Suzukawa AA;Azevedo M;Rebutini PZ;Novadzki IM;Tanabe LS;Presibella MM;Duarte Dos Santos CN
人类寨卡病毒（ZIKV）感染与先天性畸形和其他神经系统疾病（如格林-巴利综合征）有关。ZIKV宫内传播的机制、所涉及的细胞类型、感染和其他生理病理学方面严重后果的最脆弱妊娠期尚未完全阐明。在这项研究中，我们分析了在妊娠的第一、第二或第三个三个月期间从一组24名被诊断患有ZIKV感染的妇女分娩时获得的胎盘样品。绒毛不成熟是胎盘组织中的主要组织学发现，尽管也经常观察到无变化的胎盘。在妊娠晚期期间感染的女性的胎盘中观察到合胞体芽的数量显著增加，表明ZIKV感染后胎盘异常的发展。在这些晚期胎盘组织中也观察到Hofbauer细胞（HC）的增生，并且值得注意的是，HC是在所研究的胎盘中发现的唯一ZIKV阳性胎儿细胞，其持续到出生，如通过免疫组织化学（IHC）分析所揭示的。33%的妊娠期感染妇女分娩的婴儿患有先天性畸形，尽管没有观察到与感染时的妊娠期、胎盘组织中HC的IHC阳性和出生时先天性畸形的存在相关的模式。胎盘组织分析使我们能够在无法获得来自急性感染期的血清的情况下确认母体ZIKV感染，这加强了该技术在鉴定出生缺陷的可能因果因素中的重要性。我们在来自自然感染的孕妇的样品中观察到的结果可能有助于理解ZIKV的病理生理学的某些方面。
Zika virus (ZIKV) infection in humans has been associated with congenital malformations and other neurological disorders, such as Guillain-Barré syndrome. The mechanism(s) of ZIKV intrauterine transmission, the cell types involved, the most vulnerable period of pregnancy for severe outcomes from infection and other physiopathological aspects are not completely elucidated. In this study, we analyzed placental samples obtained at the time of delivery from a group of 24 women diagnosed with ZIKV infection during the first, second or third trimesters of pregnancy. Villous immaturity was the main histological finding in the placental tissues, although placentas without alterations were also frequently observed. Significant enhancement of the number of syncytial sprouts was observed in the placentas of women infected during the third trimester, indicating the development of placental abnormalities after ZIKV infection. Hyperplasia of Hofbauer cells (HCs) was also observed in these third-trimester placental tissues, and remarkably, HCs were the only ZIKV-positive fetal cells found in the placentas studied that persisted until birth, as revealed by immunohistochemical (IHC) analysis. Thirty-three percent of women infected during pregnancy delivered infants with congenital abnormalities, although no pattern correlating the gestational stage at infection, the IHC positivity of HCs in placental tissues and the presence of congenital malformations at birth was observed. Placental tissue analysis enabled us to confirm maternal ZIKV infection in cases where serum from the acute infection phase was not available, which reinforces the importance of this technique in identifying possible causal factors of birth defects. The results we observed in the samples from naturally infected pregnant women may contribute to the understanding of some aspects of the pathophysiology of ZIKV.
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影响因子：
30.3
作者：
Miner JJ;Diamond MS
通讯作者：
Diamond MS
DOI：
10.1111/aji.12613
发表时间：
2017-02
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Simoni MK;Jurado KA;Abrahams VM;Fikrig E;Guller S
通讯作者：
Guller S
影响因子：
33.9
作者：
Martines, Roosecelis Brasil;Bhatnagar, Julu;Zaki, Sherif
通讯作者：
Zaki, Sherif
DOI：
10.1056/nejmoa1602412
发表时间：
2016-12-15
期刊：
The New England journal of medicine
影响因子：
--
作者：
Brasil P;Pereira JP Jr;Moreira ME;Ribeiro Nogueira RM;Damasceno L;Wakimoto M;Rabello RS;Valderramos SG;Halai UA;Salles TS;Zin AA;Horovitz D;Daltro P;Boechat M;Raja Gabaglia C;Carvalho de Sequeira P;Pilotto JH;Medialdea-Carrera R;Cotrim da Cunha D;Abreu de Carvalho LM;Pone M;Machado Siqueira A;Calvet GA;Rodrigues Baião AE;Neves ES;Nassar de Carvalho PR;Hasue RH;Marschik PB;Einspieler C;Janzen C;Cherry JD;Bispo de Filippis AM;Nielsen-Saines K
通讯作者：
Nielsen-Saines K
影响因子：
5.2
作者：
Rabelo, Kissila;Souza, Luiz J.;Paes, Marciano, V
通讯作者：
Paes, Marciano, V