Mesenchymal stem cell-cardiomyocyte interactions under defined contact modes on laser-patterned biochips.
Mesenchymal stem cell-cardiomyocyte interactions under defined contact modes on laser-patterned biochips.
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DOI：
10.1371/journal.pone.0056554
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gao BZ
中科院分区：
文献类型：
--
作者：
Ma Z;Yang H;Liu H;Xu M;Runyan RB;Eisenberg CA;Markwald RR;Borg TK;Gao BZ
Understanding how stem cells interact with cardiomyocytes is crucial for cell-based therapies to restore the cardiomyocyte loss that occurs during myocardial infarction and other cardiac diseases. It has been thought that functional myocardial repair and regeneration could be regulated by stem cell-cardiomyocyte contact. However, because various contact modes (junction formation, cell fusion, partial cell fusion, and tunneling nanotube formation) occur randomly in a conventional coculture system, the particular regulation corresponding to a specific contact mode could not be analyzed. In this study, we used laser-patterned biochips to define cell-cell contact modes for systematic study of contact-mediated cellular interactions at the single-cell level. The results showed that the biochip design allows defined stem cell-cardiomyocyte contact-mode formation, which can be used to determine specific cellular interactions, including electrical coupling, mechanical coupling, and mitochondria transfer. The biochips will help us gain knowledge of contact-mediated interactions between stem cells and cardiomyocytes, which are fundamental for formulating a strategy to achieve stem cell-based cardiac tissue regeneration.
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影响因子：
9
作者：
Ma Z;Pirlo RK;Wan Q;Yun JX;Yuan X;Xiang P;Borg TK;Gao BZ
通讯作者：
Gao BZ
影响因子：
2.8
作者：
Ma, Zhen;Liu, Qiuying;Liu, Honghai;Yang, Huaxiao;Yun, Julie X.;Xu, Meifeng;Eisenberg, Carol A.;Borg, Thomas K.;Markwald, Roger;Gao, Bruce Z.
通讯作者：
Gao, Bruce Z.
影响因子：
64.8
作者：
Alvarez-Dolado, M;Pardal, R;Alvarez-Buylla, A
通讯作者：
Alvarez-Buylla, A
影响因子：
20.1
作者：
Kajstura, J;Rota, M;Anversa, P
通讯作者：
Anversa, P
DOI：
10.1073/pnas.0903089106
发表时间：
2009-10-06
影响因子：
11.1
作者：
Hosoda, Toru;D'Amario, Domenico;Leri, Annarosa
通讯作者：
Leri, Annarosa