Regulation of the C. elegans molt by pqn-47.
Regulation of the C. elegans molt by pqn-47.
复制标题
DOI：
10.1016/j.ydbio.2011.09.025
复制
发表时间：
2011-12-15
影响因子：
2.7
通讯作者：
Ruvkun G
中科院分区：
文献类型：
--
作者：
Russel S;Frand AR;Ruvkun G
线虫在其四个幼虫阶段的每一个阶段结束时都会蜕皮，但这个周期在繁殖的成虫阶段停止。我们已经确定了蜕皮的调节因子 pqn-47。 pqn-47 的无效突变导致幼虫第一次蜕皮时发育停滞，表明该基因活性是过渡蜕皮所必需的。具有pqn-47弱等位基因的突变体完成幼虫蜕皮，但在成虫阶段未能退出蜕皮周期。这些表型表明 pqn-47 执行蜕皮程序的关键方面，包括停止蜕皮周期。 pqn-47 基因编码的蛋白质在动物系统发育中高度保守，但由于与酵母转录因子的同源性不太明显，可能在基因组序列中被错误注释。 PQN-47∷GFP 融合基因在许多神经元、外阴前体细胞、远端细胞 (DTC)、肠和外侧皮下缝细胞中表达，但不在位于、合成和释放大部分胶原角质层之下的主体皮下合胞体 (hyp7) 中表达。功能性 PQN-47∷GFP 融合蛋白在所有发育阶段都定位于细胞质而不是细胞核，包括蜕皮之前和期间的时期，此时遗传分析表明 pqn-47 发挥作用。 PQN-47∷GFP 的细胞质定位与内质网部分重叠，表明 PQN-47 参与蜕皮过程中发生的角质层成分或激素的广泛分泌。 pqn-47 的哺乳动物和昆虫同源物可能在调节分泌中发挥相似的作用。
C. elegans molts at the end of each of its four larval stages but this cycle ceases at the reproductive adult stage. We have identified a regulator of molting, pqn-47. Null mutations in pqn-47 cause a developmental arrest at the first larval molt, showing that this gene activity is required to transit the molt. Mutants with weak alleles of pqn-47 complete the larval molts but fail to exit the molting cycle at the adult stage. These phenotypes suggest that pqn-47 executes key aspects of the molting program including the cessation of molting cycles. The pqn-47 gene encodes a protein that is highly conserved in animal phylogeny but probably misannotated in genome sequences due to much less significant homology to a yeast transcription factor. A PQN-47∷GFP fusion gene is expressed in many neurons, vulval precursor cells, the distal tip cell (DTC), intestine, and the lateral hypodermal seam cells but not in the main body hypodermal syncytium (hyp7) that underlies, synthesizes, and releases most of the collagenous cuticle. A functional PQN-47∷GFP fusion protein localizes to the cytoplasm rather than the nucleus at all developmental stages, including the periods preceding and during ecdysis when genetic analysis suggests that pqn-47 functions. The cytoplasmic localization of PQN-47∷GFP partially overlaps with the endoplasmic reticulum, suggesting that PQN-47 is involved in the extensive secretion of cuticle components or hormones that occurs during molts. The mammalian and insect homologues of pqn-47 may serve similar roles in regulated secretion.
登录
查看更多内容
影响因子：
2.7
作者：
HEDGECOCK, EM;CULOTTI, JG;PERKINS, LA
通讯作者：
PERKINS, LA
影响因子：
9.2
作者：
Caygill, Elizabeth E.;Johnston, Laura A.
通讯作者：
Johnston, Laura A.
影响因子：
64.5
作者：
Emery B;Agalliu D;Cahoy JD;Watkins TA;Dugas JC;Mulinyawe SB;Ibrahim A;Ligon KL;Rowitch DH;Barres BA
通讯作者：
Barres BA
影响因子：
56.9
作者：
AMBROS, V;HORVITZ, HR
通讯作者：
HORVITZ, HR
影响因子：
5.3
作者：
Hong, Y;Lee, RC;Ambros, V
通讯作者：
Ambros, V