Preharvest Hydrogen Peroxide Treatment Delays Leaf Senescence of Chinese Flowering Cabbage During Storage by Reducing Water Loss and Activating Antioxidant Defense System.
Preharvest Hydrogen Peroxide Treatment Delays Leaf Senescence of Chinese Flowering Cabbage During Storage by Reducing Water Loss and Activating Antioxidant Defense System.
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采前过氧化氢处理通过减少水分流失和激活抗氧化防御系统延缓大白菜贮藏期间叶片衰老
DOI：
10.3389/fpls.2022.856646
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
白菜采后叶片黄化是白菜衰老的一个指标，降低了菜心的商品价值。过氧化氢（H2O2）在介导植物胁迫反应中起着双重作用，但在收获前外源刺激植物时，它是否以及如何影响叶片衰老尚不清楚。在这里，我们发现，收获前应用低浓度的H2O2根延迟叶片衰老。在贮藏4天和8天后，相对于对照，约10 mM H2O2分别使叶片黄化率降低8.2和26.4%。与对照相比，H2O2处理的甘蓝叶绿素含量增加，衰老相关基因BrSAG12的相对表达量降低。蛋白质组学分析显示，118和204差异表达的蛋白质（DEPs）在过氧化氢处理的植物在4和8天的存储，分别。DEPs主要参与叶绿素的降解和合成、水分剥夺、抗氧化活性和叶绿体膜的保护。H_2O_2处理降低了白菜叶片失水量，增加了脯氨酸含量，调节了叶片气孔开度。通过抑制呼吸爆发氧化酶同源物和上调活性氧（ROS）清除相关基因来减轻氧化应激。H_2O_2处理组的脂质过氧化减轻，细胞完整性和光化学效率的保护也支持了这些结果。采前H_2O_2处理可抑制叶片失水，激活抗氧化防御系统，保护叶绿体膜免受氧化损伤，延缓叶片衰老。本研究为H2O2调控菜心叶片衰老提供了新的思路。
Leaf yellowing, an indicator of senescence, reduces commercial value of Chinese flowering cabbage after harvest. Hydrogen peroxide (H2O2) plays a dual role in mediating plant stress responses, but it is not clear whether and how it affects leaf senescence when exogenously stimulating the plants before harvest. Here, we found that preharvest application with low concentrations of H2O2 to root delays leaf senescence. Around 10 mM H2O2 reduced leaf yellowing rate by 8.2 and 26.4% relative to the control following 4 and 8 days storage, respectively. The H2O2-treated cabbages showed higher chlorophyll and lower relative expression of senescence-associated gene (SAG) BrSAG12 than the control. Proteomic analysis revealed 118 and 204 differentially expressed proteins (DEPs) in H2O2-treated plants at 4 and 8 days of storage, respectively. The main DEPs are involved in chlorophyll degradation and synthesis, water deprivation, antioxidant activity, and protections on chloroplast membranes. Decline of water loss in H2O2-treated cabbages was coincide with increase of proline contents and modulation of leaf stomatal aperture. Alleviation of oxidative stress was indicated by suppression of respiratory burst oxidase homolog and upregulation of reactive oxygen species (ROS) scavenging-related genes. These results were also supported by the alleviation of lipid peroxidation and the protections on cell integrity and photochemical efficiency in H2O2-treated group. Collectively, preharvest H2O2 treatment alleviates water loss and activates antioxidant defense system, protects chloroplast membrane from oxidative damage, and ultimately delays leaf senescence during storage. This study provides novel insights into the roles of H2O2 for regulating leaf senescence of Chinese flowering cabbage.
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影响因子：
4.8
作者：
Hanif, Sajid;Saleem, Muhammad Farrukh;Khan, Haroon Zaman
通讯作者：
Khan, Haroon Zaman
DOI：
10.1111/pce.13195
发表时间：
2019-01
期刊：
Plant, cell & environment
影响因子：
--
作者：
Khan N;Bano A;Rahman MA;Rathinasabapathi B;Babar MA
通讯作者：
Babar MA
影响因子：
16.6
作者：
Exposito-Rodriguez M;Laissue PP;Yvon-Durocher G;Smirnoff N;Mullineaux PM
通讯作者：
Mullineaux PM
ShDHN（来自 Solanum habrochaites 的脱水蛋白基因）的过度表达可增强番茄对多种非生物胁迫的耐受性
影响因子：
5.2
作者：
Liu, Hui;Yu, Chuying;Ye, Zhibiao
通讯作者：
Ye, Zhibiao
影响因子：
5.2
作者：
Rosenwasser, S;Mayak, S;Friedman, H
通讯作者：
Friedman, H