Birth, survival and differentiation of neurons in an adult crustacean brain.
Birth, survival and differentiation of neurons in an adult crustacean brain.
复制标题
DOI：
10.1002/dneu.22156
复制
发表时间：
2014-06
影响因子：
3
通讯作者：
Beltz, Barbara S.
中科院分区：
文献类型：
--
作者：
Kim, Youngmi Faith;Sandeman, David C.;Benton, Jeanne L.;Beltz, Barbara S.
终生神经发生是许多脊椎动物和无脊椎动物的特征。在十足目甲壳类动物中，新的神经元在整个生命周期中都会添加到嗅觉通路中包含局部（簇 9）和投射（簇 10）中间神经元的两个细胞簇中。小龙虾中第 9 簇和第 10 簇的成年神经元在出生后 6 个月时具有成熟神经元的解剖学特性和化学性质。在这里，我们使用 5-溴-2'-脱氧尿苷 (BrdU) 掺入来脉冲标记这些细胞簇中有丝分裂活性的细胞，然后存活时间长达 8 个月，在此期间每隔一定时间处死小龙虾 (Cherax destructor)，并对 BrdU 标记的细胞数量进行定量。我们发现，在暴露于 BrdU 后的第一周和第二周之间，细胞簇 10 中 BrdU 标记的细胞数量有所减少，这表明细胞在增殖后不久就会死亡一段时间。在 BrdU 清除时间后很长一段时间，标记细胞的增加表明两个细胞簇中额外的细胞分裂延迟。检测簇 10 BrdU 标记细胞中递质 SIFamide 的首次免疫反应性用于确定这些细胞分化为神经元的时间，该时间从 BrdU 标记后 4 周开始；在接下来的一个月里，SIFamide 标记的细胞数量继续增加。测试 Cluster 10 细胞的增殖和存活是否受运动活动影响的实验没有提供活动水平与细胞增殖之间相关性的证据，但表明运动活动对细胞存活有强烈影响。
Life-long neurogenesis is a characteristic feature of many vertebrate and invertebrate species. In decapod crustaceans, new neurons are added throughout life to two cell clusters containing local (cluster 9) and projection (cluster 10) interneurons in the olfactory pathway. Adult-born neurons in clusters 9 and 10 in crayfish have the anatomical properties and chemistry of mature neurons by 6 months after birth. Here we use 5-bromo-2'-deoxyuridine (BrdU) incorporation to pulse label mitotically active cells in these cell clusters, followed by a survival time of up to 8 months, during which crayfish (Cherax destructor) were sacrificed at intervals and the numbers of BrdU-labeled cells quantified. We find a decrease in the numbers of BrdU-labeled cells in cell cluster 10 between the first and second weeks following BrdU exposure, suggesting a period of cell death shortly after proliferation. Additional delayed cell divisions in both cell clusters are indicated by increases in labeled cells long after the BrdU clearing time. Detection of the first immunoreactivity for the transmitter SIFamide in cluster 10 BrdU-labeled cells was used to define the differentiation time of these cells into neurons, which begins at 4 weeks after BrdU-labeling; the numbers of SIFamide-labeled cells continues to increase over the following month. Experiments testing whether proliferation and survival of Cluster 10 cells are influenced by locomotor activity provided no evidence of a correlation between activity levels and cell proliferation, but suggest a strong influence of locomotor activity on cell survival.
登录
查看更多内容
影响因子：
3.6
作者：
Polanska, M. A.;Yasuda, A.;Harzsch, S.
通讯作者：
Harzsch, S.
影响因子：
64.8
作者：
Kempermann, G;Kuhn, HG;Gage, FH
通讯作者：
Gage, FH
DOI：
10.1073/pnas.231471298
发表时间：
2001-10-23
影响因子：
11.1
作者：
Beltz, BS;Benton, JL;Sullivan, JM
通讯作者：
Sullivan, JM
DOI：
10.1007/bf02457638
发表时间：
1991-01-01
期刊：
ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
SANDEMAN, R;SANDEMAN, D
通讯作者：
SANDEMAN, D
影响因子：
4
作者：
Chaves da Silva, Paula Grazielle;Benton, Jeanne L.;Beltz, Barbara S.
通讯作者：
Beltz, Barbara S.