Spatial modulation of biomolecules immobilization by fabrication of hierarchically structured PEG-derived brush micropatterns: An versatile cellular microarray platform
Spatial modulation of biomolecules immobilization by fabrication of hierarchically structured PEG-derived brush micropatterns: An versatile cellular microarray platform
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通过制造分层结构的 PEG 衍生刷微图案对生物分子固定进行空间调节：多功能细胞微阵列平台
DOI：
10.1016/j.apsusc.2020.147056
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发表时间：
2020-11
影响因子：
6.7
通讯作者：
Yulu Ma
中科院分区：
文献类型：
--
作者：
Haili Zhao;Jin Sha;Tong Wu;Tao Chen;Xin Chen;Huajian Ji;Yu Wang;Huihao Zhu;Linsheng Xie;Yulu Ma
微阵列技术在从组织工程、再生医学到高通量筛查的各个领域的发展中有着巨大的前景。基于数字微镜器件(DMD)对表面引发的光诱导原子转移自由基聚合(Photo-ATRP)过程的时空调控，开发了一种灵活而通用的层次化微阵列结构，该结构由均匀的超支化聚乙二醇(PEG)衍生的刷层作为防污背景，以及方形网格聚(2-叠氮基-2-甲基-1-氧丙氧基)甲基丙烯酸乙酯(PAMEMA)的延伸层在硅衬底上刷出微图形。PAMEMA刷子侧链上的末端叠氮基团为实现RGD肽、纤维连接蛋白、牛血清白蛋白和链霉亲和素等目标生物分子的共价固定提供了丰富的活性中心。TOF-SIMS和荧光表征证明了通过调节PAMEMA刷状微图形的三维结构参数进而调控叠氮化学官能团来空间调控表面结合生物分子密度的可行性和有效性。此外，在获得的层次化微阵列结构上高通量地进行了人骨髓基质细胞和小鼠L929细胞的培养实验。提出的分层微阵列结构具有作为高通量筛选平台的良好潜力，允许并行评估细胞-表面相互作用。
Microarray technology holds enormous promise in the development of various fields ranging from tissue engineering, regenerative medicine to high-throughput screening. Here, based on the digital micromirror device (DMD)-based spatiotemporal regulation of surface-initiated photoinduced atom transfer radical polymerization (Photo-ATRP) process, a flexible and versatile methodology was developed to fabricate a hierarchical microarray structure constituted with a homogeneous hyperbranched polyethylene glycol (PEG)-derived brush layer as anti-fouling background and an extension layer of square-grid poly(2-(2-azido-2-methyl-1-oxopropoxy) ethyl methacrylate) (PAMEMA) brushes micropatterns on the silicon substrate. The terminal azido groups on the side chains of PAMEMA brushes provide abundant reactive sites to realize the covalent immobilization of target biomolecules, including RGD peptide, fibronectin, BSA and streptavidin. The TOF-SIMS and fluorescence characterizations demonstrated the feasibility and efficiency of spatially modulating the density of surface-bound biomolecules through regulating the 3D architecture parameters of the PAMEMA brush micropatterns and consequentially the azido chemical functionality. Moreover, culture experiments of human bone-derived marrow stromal cells (BMSCs) and mouse L929 cells were conducted on the obtained hierarchical microarray structure in a high-throughput manner. The presented hierarchical microarray structure holds excellent potential as a high-throughput screening platform, allowing for the parallel assessment of cell-surface interactions.
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影响因子：
14
作者：
Seo J;Shin JY;Leijten J;Jeon O;Camci-Unal G;Dikina AD;Brinegar K;Ghaemmaghami AM;Alsberg E;Khademhosseini A
通讯作者：
Khademhosseini A
通过聚合物笔光刻对复杂的 2D 和 3D 功能性聚合物刷进行大规模并行图案化
影响因子：
9.5
作者：
Xie, Zhuang;Chen, Chaojian;Zheng, Zijian
通讯作者：
Zheng, Zijian
影响因子：
--
作者：
Jory Lietard;Dominik Ameur;Masad J. Damha;Mark M. Somoza
通讯作者：
Jory Lietard;Dominik Ameur;Masad J. Damha;Mark M. Somoza
开发用于制造高性能微阵列的球聚合物刷分层纳米结构基底
影响因子：
9.5
作者：
Liu Xia;Tian Rongrong;Liu Dianjun;Wang Zhenxin
通讯作者：
Wang Zhenxin
影响因子：
6.7
作者：
Xuechang Zhou;Xuqing Liu;Zhuang Xie;Zijian Zheng
通讯作者：
Xuechang Zhou;Xuqing Liu;Zhuang Xie;Zijian Zheng