CBFB and MYH11 in inv(16)(p13q22) of acute myeloid leukemia displaying close spatial proximity in interphase nuclei of human hematopoietic stem cells.
CBFB and MYH11 in inv(16)(p13q22) of acute myeloid leukemia displaying close spatial proximity in interphase nuclei of human hematopoietic stem cells.
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急性髓样白血病的Inv（16）（P13Q22）中的CBFB和MYH11在人造血干细胞的相间核中表现出紧密的空间近端。
DOI：
10.1002/gcc.20896
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发表时间：
2011-09
影响因子：
3.7
通讯作者：
Wang, Yuh-Hwa
中科院分区：
文献类型：
--
作者：
Weckerle, Allison B.;Santra, Madhumita;Ng, Maggie C. Y.;Koty, Patrick P.;Wang, Yuh-Hwa
To gain a better understanding of the mechanism of chromosomal translocations in cancer, we investigated the spatial proximity between CBFB and MYH11 genes involved in inv(16)(p13q22) found in acute myeloid leukemia patients. Previous studies have demonstrated a role for spatial genome organization in the formation of tumorigenic abnormalities. The non-random localization of chromosomes and, more specifically, of genes appears to play a role in the mechanism of chromosomal translocations. Here, two-color fluorescence in situ hybridization and confocal microscopy were used to measure the interphase distance between CBFB and MYH11 in hematopoietic stem cells, where inv(16)(p13q22) is believed to occur, leading to leukemia development. The measured distances in hematopoietic stem cells were compared to mesenchymal stem cells, peripheral blood lymphocytes and fibroblasts, as spatial genome organization is determined to be cell-type specific. Results indicate that CBFB and MYH11 are significantly closer in hematopoietic stem cells compared to all other cell types examined. Furthermore, the CBFB-MYH11 distance is significantly reduced compared to CBFB and a control locus in hematopoietic stem cells, although separation between CBFB and the control is ~70% of that between CBFB and MYH11 on metaphase chromosomes. Hematopoietic stem cells were also treated with fragile site-inducing chemicals since both genes contain translocation breakpoints within these regions. However, treatment with fragile site-inducing chemicals did not significantly affect the interphase distance. Consistent with previous studies, our results suggest that gene proximity may play a role in the formation of cancer-causing rearrangements, providing insight into the mechanism of chromosomal abnormalities in human tumors.
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影响因子：
64.5
作者：
Casper, AM;Nghiem, P;Glover, TW
通讯作者：
Glover, TW
DOI：
10.1126/science.1178124
发表时间：
2009-11-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Mani RS;Tomlins SA;Callahan K;Ghosh A;Nyati MK;Varambally S;Palanisamy N;Chinnaiyan AM
通讯作者：
Chinnaiyan AM
影响因子：
21.3
作者：
Dimitrova, DS;Gilbert, DM
通讯作者：
Gilbert, DM
影响因子：
64.5
作者：
Castilla, LH;Wijmenga, C;Liu, PP
通讯作者：
Liu, PP
影响因子：
4
作者：
Otto, F;Lübbert, M;Stock, M
通讯作者：
Stock, M