Aeromonas hydrophila RIT668 and Citrobacter portucalensis RIT669-Potential Zoonotic Pathogens Isolated from Spotted Turtles.
Aeromonas hydrophila RIT668 and Citrobacter portucalensis RIT669-Potential Zoonotic Pathogens Isolated from Spotted Turtles.
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嗜水气单胞菌RIT668和柠檬酸杆菌RIT669-从斑点甲鱼中分离的潜在人畜共患病病原体
DOI：
10.3390/microorganisms8111805
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发表时间：
2020-11-17
期刊：
影响因子：
4.5
通讯作者：
Hudson AO
中科院分区：
文献类型：
--
作者：
Thomas SG;Abajorga M;Glover MA;Wengert PC;Parthasarathy A;Savka MA;Wadsworth CB;Shipman PA;Hudson AO
抗菌素耐药性（AMR）是21世纪世纪面临的最大挑战之一，生物膜的形成使细菌能够在比嗜酸细胞高得多的浓度下抵抗抗生素。之前，我们发现革兰氏阴性气单胞菌（AeromonaseriaRIT 668）和柠檬酸杆菌（CitrobacterportucalensisRIT 669）（与C. freundii NBRC 12681）在塑料表面形成生物膜并上调毒素表达，并预测其具有多种抗生素抗性基因。在这里，我们表明，他们每个人都能抵抗几种抗生素的反硝化阶段，但对新霉素，高浓度的四环素和复方新诺明敏感。使用卡尔加里装置测试它们的生物膜对新霉素和复方新诺明的敏感性。对于.复方新诺明的最低抑菌浓度（MIC）为500-1000，最低生物膜清除浓度（MBEC）> 1000 μg/mL;新霉素的MIC为32.3-62.5，MBEC > 1000 μg/mL。梭复方新诺明对弗氏菌的MIC为7.8-15.6，MBEC > 1000 μg/mL;新霉素对弗氏菌的MIC为7.8，MBEC > 1000 μg/mL。A.和C. portucalensis激活了一个酰基高丝氨酸内酯（阿勒）依赖的生物传感器，表明群体感应可以介导生物膜的形成。它们的多药耐药性以抗生素形式存在，即使用新霉素和复方新诺明也很难根除生物膜，这表明A。和C. portucalensis是潜在的人畜共患病原体，对植入植入物的患者有风险。
Antimicrobial resistance (AMR) is one of the biggest challenges of the 21st century, and biofilm formation enables bacteria to resist antibiotic at much higher concentrations than planktonic cells. Earlier, we showed that the Gram-negative Aeromonas hydrophila RIT668 and Citrobacter portucalensis RIT669 (closely related to C. freundii NBRC 12681) from infected spotted turtles (Clemmys guttata), formed biofilms and upregulated toxin expression on plastic surfaces, and were predicted to possess multiple antibiotic resistance genes. Here, we show that they each resist several antibiotics in the planktonic phase, but were susceptible to neomycin, and high concentrations of tetracycline and cotrimoxazole. The susceptibility of their biofilms to neomycin and cotrimoxazole was tested using the Calgary device. For A. hydrophila, the minimum inhibitory concentration (MIC) = 500–1000, and the minimum biofilm eradication concentration (MBEC) > 1000 μg/mL, using cotrimoxazole, and MIC = 32.3–62.5, and MBEC > 1000 μg/mL, using neomycin. For C. freundii MIC = 7.8–15.6, and, MBEC > 1000 μg/mL, using cotrimoxazole, and MIC = 7.8, and MBEC > 1000 μg/mL, using neomycin. Both A. hydrophila and C. portucalensis activated an acyl homoserine lactone (AHL) dependent biosensor, suggesting that quorum sensing could mediate biofilm formation. Their multidrug resistance in the planktonic form, and weak biofilm eradication even with neomycin and cotrimoxazole, indicate that A. hydrophila and C. portucalensis are potential zoonotic pathogens, with risks for patients living with implants.
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影响因子：
3.3
作者：
Al-Bahry, Saif N.;Mahmoud, Ibrahim Y.;Al-Alawi, Wafaa
通讯作者：
Al-Alawi, Wafaa
影响因子：
9.4
作者：
Ceri, H;Olson, ME;Buret, A
通讯作者：
Buret, A
影响因子：
4.6
作者：
Anderson MT;Mitchell LA;Zhao L;Mobley HLT
通讯作者：
Mobley HLT
影响因子：
3.1
作者：
Bernier, SP;Silo-Suh, L;Sokol, PA
通讯作者：
Sokol, PA
DOI：
10.1007/s10096-007-0308-2
发表时间：
2007-08-01
影响因子：
4.5
作者：
Choi, S.-H.;Lee, J. E.;Kim, Y. S.
通讯作者：
Kim, Y. S.