A systematic comparison of embryogenic and non-embryogenic cells of banana (Musa spp. AAA): Ultrastructural, biochemical and cell wall component analyses
A systematic comparison of embryogenic and non-embryogenic cells of banana (Musa spp. AAA): Ultrastructural, biochemical and cell wall component analyses
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香蕉（Musa spp. AAA）胚胎发生和非胚胎发生细胞的系统比较：超微结构、生化和细胞壁成分分析
DOI：
10.1016/j.scienta.2013.05.008
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发表时间：
2013-07
影响因子：
4.3
通讯作者：
Xu, Chunxiang
中科院分区：
文献类型：
--
作者：
Wu, Xiaoying;Takac, Tomas;Samaj, Jozef;Xu, Chunxiang
植株再生体细胞胚胎发生是无性繁殖香蕉(Musaspp.AAA)通过生物技术。对香蕉胚性细胞和非胚性细胞进行了系统比较。通过免疫斑点印迹、免疫荧光标记、生化和超微结构分析研究ECs和NEC的差异，免疫斑点分析和免疫荧光标记均显示ECs细胞壁中岩藻糖化木聚糖含量较高。LM2和NH4.3E5抗体识别的阿拉伯半乳糖蛋白(AGP)表位在NECs中更为丰富。另一方面，JIM16、MAC204和MAC207抗体识别的AGPs在内皮细胞中更为丰富。生化分析表明，ECS的总可溶性糖和还原糖含量以及吲哚-3-乙酸、脱落酸和玉米素核苷的含量均显著高于NECS。相反，NECS的赤霉酸含量显著高于NECS。此外，NECs高度液泡化，含有脂体、突出的线粒体和叶绿体，而内质网和高尔基体不如ECs丰富。本研究发现，NECs的细胞壁富含岩藻糖化的木葡聚糖和JIM16、MAC204和MAC207抗体标记的AGP表位，表明这些细胞壁成分与较高水平的生长素、脱落酸和玉米素核苷一起参与了香蕉的胚胎发生能力。相反，NECS的细胞壁中非岩藻糖化和非XXXG木聚糖含量较高，赤霉酸含量较高。本研究提供了有关激素和细胞壁调控香蕉胚性的新的有用信息，有助于更好地理解这一过程，并可能在不久的将来导致实际应用。
Plant regenerationviasomatic embryogenesis is essential for improvement of vegetatively propagated banana (Musaspp. AAA) through biotechnological techniques. A systematic comparison of embryogenic cells (ECs) and non-embryogenic cells (NECs) of banana was performed in the present study. Immuno-dot blotting, immunoflourescence labeling, biochemical and ultrastructural analyses were employed to study differences between ECs and NECs.Both immuno-dot analysis and immunofluorescence labeling revealed a higher abundance of fucosylated xyloglucans in cell walls of ECs. Arabinogalactan-protein (AGP) epitopes recognized by LM2 and NH4.3E5 antibodies were more abundant in NECs. On the other hand, AGPs recognized by JIM16, MAC204 and MAC207 antibodies were more abundant in ECs. Biochemical analyses revealed that the content of total soluble sugars and reducing sugars, as well as levels of indole-3-acetic acid, abscisic acid and zeatin riboside were significantly higher in ECs in comparison to NECs. In contrast, NECs showed significantly higher content of gibberellic acid. In addition, NECs were highly vacuolated and contained lipid bodies, prominent mitochondria and plastids, however, endoplasmic reticulum and Golgi bodies were not so abundant when compared to ECs.This study revealed that cell walls of ECs are rich in fucosylated xyloglucan and in AGP epitopes labeled by JIM16, MAC204 and MAC207 antibodies, indicating that these cell wall components together with higher levels of auxin, abscisic acid and zeatin riboside are contributing to the embryogenic competence of banana. In contrast, NECs showed more abundant non-fucosylated and non-XXXG xyloglucans in their cell walls, and higher level of gibberellic acid. This study provides new and useful information about hormonal and cell wall regulation of banana embryogenic competence which contributes to better understanding of this process, and might lead to practical applications in the near future.
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影响因子：
4.6
作者：
S. Perotto;K. VandenBosch;G. Butcher;N. J. Brewin
通讯作者：
S. Perotto;K. VandenBosch;G. Butcher;N. J. Brewin
影响因子：
3.7
作者：
Xu C;Zhao L;Pan X;Samaj J
通讯作者：
Samaj J
影响因子：
6.9
作者：
M. Esquerré-Tugayé;D. Mazau
通讯作者：
M. Esquerré-Tugayé;D. Mazau
影响因子：
6.2
作者：
Konieczny, Robert;Swierczynska, Joanna;Bohdanowicz, Jerzy
通讯作者：
Bohdanowicz, Jerzy
影响因子：
2.6
作者：
Moghaddam, BE;Taha, RM
通讯作者：
Taha, RM