Developmental localization and methylesterification of pectin epitopes during somatic embryogenesis of banana (Musa spp. AAA).
Developmental localization and methylesterification of pectin epitopes during somatic embryogenesis of banana (Musa spp. AAA).
复制标题
DOI：
10.1371/journal.pone.0022992
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Samaj J
中科院分区：
文献类型：
--
作者：
Xu C;Zhao L;Pan X;Samaj J
植物细胞壁在体细胞胚胎发生和植物发育中起着重要作用。果胶是初级细胞壁的主要化学成分，而同型半乳糖醛酸（HG）是最丰富的果胶多糖。 HG 甲基酯化程度的发育调节对于细胞粘附、分裂和扩张以及通常对于适当的器官和植物发育很重要。研究了香蕉（Musa spp. AAA）体细胞胚胎发生过程中果胶同型半乳糖醛酸（HG）表位和鼠李糖半乳糖醛酸I（RG-I）的（1→4）-β-D-半乳聚糖表位的发育定位以及果胶甲基酯化（DM）的程度。组织学分析记录了所有主要发育阶段，包括胚胎发生细胞（EC）、球状前、球状、梨形和子叶体细胞胚胎。用钌红对细胞外分泌的果胶进行组织化学染色显示，前球形、球形和梨形体细胞胚胎表面的染色最强烈。生化分析揭示了不同胚胎发生阶段半乳糖醛酸含量和 DM 的发育调节。组织切片上的免疫点和免疫标记揭示了体细胞胚胎发生过程中 JIM7 和 LM20 抗体识别的高度甲基酯化的 HG 表位的发育调节。球状前/球状和晚期胚胎的细胞壁含有低甲基酯化的HG表位以及部分和高度甲基酯化的表位。覆盖早期发育胚胎表面的细胞外基质含有可被 2F4、LM18、JIM5、JIM7 和 LM5 抗体识别的果胶表位。对于高度甲基酯化的 HG 表位，NaOH 对细胞壁果胶的去酯化作用导致免疫标记的减少或消除。然而，一些低甲基酯化表位的免疫标记在这种基础处理后显得更强。这些数据表明，低甲基酯化和高度甲基酯化的HG表位在体细胞胚胎发生过程中的不同胚胎发生阶段均受到发育调节。本研究提供了香蕉体细胞胚胎发生过程中果胶组成、HG 甲基酯化和果胶表位发育定位的新信息。
The plant cell walls play an important role in somatic embryogenesis and plant development. Pectins are major chemical components of primary cell walls while homogalacturonan (HG) is the most abundant pectin polysaccharide. Developmental regulation of HG methyl-esterification degree is important for cell adhesion, division and expansion, and in general for proper organ and plant development. Developmental localization of pectic homogalacturonan (HG) epitopes and the (1→4)-β-D-galactan epitope of rhamnogalacturonan I (RG-I) and degree of pectin methyl-esterification (DM) were studied during somatic embryogenesis of banana (Musa spp. AAA). Histological analysis documented all major developmental stages including embryogenic cells (ECs), pre-globular, globular, pear-shaped and cotyledonary somatic embryos. Histochemical staining of extracellularly secreted pectins with ruthenium red showed the most intense staining at the surface of pre-globular, globular and pear-shaped somatic embryos. Biochemical analysis revealed developmental regulation of galacturonic acid content and DM in diverse embryogenic stages. Immunodots and immunolabeling on tissue sections revealed developmental regulation of highly methyl-esterified HG epitopes recognized by JIM7 and LM20 antibodies during somatic embryogenesis. Cell walls of pre-globular/globular and late-stage embryos contained both low methyl-esterified HG epitopes as well as partially and highly methyl-esterified ones. Extracellular matrix which covered surface of early developing embryos contained pectin epitopes recognized by 2F4, LM18, JIM5, JIM7 and LM5 antibodies. De-esterification of cell wall pectins by NaOH caused a decrease or an elimination of immunolabeling in the case of highly methyl-esterified HG epitopes. However, immunolabeling of some low methyl-esterified epitopes appeared stronger after this base treatment. These data suggest that both low- and highly-methyl-esterified HG epitopes are developmentally regulated in diverse embryogenic stages during somatic embryogenesis. This study provides new information about pectin composition, HG methyl-esterification and developmental localization of pectin epitopes during somatic embryogenesis of banana.
登录
查看更多内容
影响因子：
6.9
作者：
Bárány I;Fadón B;Risueño MC;Testillano PS
通讯作者：
Testillano PS
DOI：
10.1139/cjb-78-6-824
发表时间：
2000-06-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
Chapman, A;Blervacq, AS;Hilbert, JL
通讯作者：
Hilbert, JL
影响因子：
7.4
作者：
LINERS, F;LETESSON, JJ;VANCUTSEM, P
通讯作者：
VANCUTSEM, P
影响因子：
6.2
作者：
Konieczny, Robert;Swierczynska, Joanna;Bohdanowicz, Jerzy
通讯作者：
Bohdanowicz, Jerzy
影响因子：
2.9
作者：
LINERS, F;VANCUTSEM, P
通讯作者：
VANCUTSEM, P