A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.).
A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.).
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水稻早期种子发育的定量乙酰化分析（Oryza sativa L.）
DOI：
10.3390/ijms18071376
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发表时间：
2017-06-27
影响因子：
5.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Wang Y;Hou Y;Qiu J;Li Z;Zhao J;Tong X;Zhang J
PKA(蛋白质赖氨酸乙酰化)是一种关键的翻译后修饰，调节包括种子发育在内的各种发育过程。然而，这一过程中蛋白质组水平上的乙酰化事件和动态仍然很大程度上是未知的，特别是在水稻种子发育的早期。我们报道了首次利用基于质谱学的无标记方法对水稻早期种子发育进行的定量乙酰化蛋白质组学研究。在授粉后第3天和第7天，在雌蕊和种子中共鉴定出972个乙酰化蛋白中的1688个乙酰肽上的1817个乙酰化位点，其中268个乙酰化蛋白在三个时期之间存在差异。Motif-X分析表明，在鉴定的水稻种子乙酰化蛋白的乙酰基周围有6个显著富含的基序，如(DxkK)、(KH)和(Ky)。三个阶段乙酰化蛋白的差异，包括二磷酸腺苷(ADP)-葡萄糖焦磷酸化酶(AGPs)、PDIL1-1(类蛋白质二硫键异构酶1-1)、己糖激酶酶、丙酮酸脱氢酶复合体(PDC)和许多其他调节因子，它们广泛参与种子发育早期的淀粉和蔗糖代谢、糖酵解/糖异生、三羧酸循环和光合作用途径。这项研究极大地扩展了水稻乙酰组数据集，并对PKA在水稻种子早期发育中的调控作用提供了新的见解。
PKA (protein lysine acetylation) is a critical post-translational modification that regulates various developmental processes, including seed development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early seed development. We report the first quantitative acetylproteomic study focused on rice early seed development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early seed development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early seed development.
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影响因子：
3.7
作者：
Nallamilli BR;Edelmann MJ;Zhong X;Tan F;Mujahid H;Zhang J;Nanduri B;Peng Z
通讯作者：
Peng Z
影响因子：
5.6
作者：
Fang X;Chen W;Zhao Y;Ruan S;Zhang H;Yan C;Jin L;Cao L;Zhu J;Ma H;Cheng Z
通讯作者：
Cheng Z
影响因子：
14.9
作者：
Mitchell A;Chang HY;Daugherty L;Fraser M;Hunter S;Lopez R;McAnulla C;McMenamin C;Nuka G;Pesseat S;Sangrador-Vegas A;Scheremetjew M;Rato C;Yong SY;Bateman A;Punta M;Attwood TK;Sigrist CJ;Redaschi N;Rivoire C;Xenarios I;Kahn D;Guyot D;Bork P;Letunic I;Gough J;Oates M;Haft D;Huang H;Natale DA;Wu CH;Orengo C;Sillitoe I;Mi H;Thomas PD;Finn RD
通讯作者：
Finn RD
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
7.4
作者：
Finkemeier, Iris;Laxa, Miriam;Sweetlove, Lee J.
通讯作者：
Sweetlove, Lee J.