Effects of the insemination of hydrogen peroxide-treated epididymal mouse spermatozoa on γH2AX repair and embryo development.
Effects of the insemination of hydrogen peroxide-treated epididymal mouse spermatozoa on γH2AX repair and embryo development.
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DOI：
10.1371/journal.pone.0038742
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xiao W
中科院分区：
文献类型：
--
作者：
Xiao J;Liu Y;Li Z;Zhou Y;Lin H;Wu X;Chen M;Xiao W
由于活性氧（ROS）的过量产生会对精子造成低温损伤，因此辅助生殖用人类精液的低温保存变得复杂。我们使用外源ROS （H2O2）模拟低温保存，并检测了H2O2诱导DNA损伤的精子受精胚胎的DNA损伤修复。取成年雄性KM小鼠附睾精子，分别用获能培养基（含0、0.1、0.5、1 mM H2O2）或冷冻保存。以精子活力、活力和DNA损伤修复标志物γ - h2ax的表达为基础建立DNA损伤精子模型。我们检测了dna受损精子受精胚胎的受精率、发育、细胞分裂和γ - h2ax水平。低温保存和1mm H2O2处理产生类似的DNA损伤。大多数与dna受损的精子受精的单细胞和双细胞胚胎在囊胚阶段之前显示出卵裂延迟。免疫细胞化学在一细胞和四细胞胚胎中发现γ - h2ax。γ - h2ax可能参与了受氧胁迫精子受精的着床前胚胎的修复。
Cryopreservation of human semen for assisted reproduction is complicated by cryodamage to spermatozoa caused by excessive reactive oxygen species (ROS) generation. We used exogenous ROS (H2O2) to simulate cryopreservation and examined DNA damage repair in embryos fertilized with sperm with H2O2-induced DNA damage. Sperm samples were collected from epididymis of adult male KM mice and treated with capacitation medium (containing 0, 0.1, 0.5 and 1 mM H2O2) or cryopreservation. The model of DNA-damaged sperm was based on sperm motility, viability and the expression of γH2AX, the DNA damage-repair marker. We examined fertility rate, development, cell cleavage, and γH2AX level in embryos fertilized with DNA-damaged sperm. Cryopreservation and 1-mM H2O2 treatment produced similar DNA damage. Most of the one- and two-cell embryos fertilized with DNA-damaged sperm showed a delay in cleavage before the blastocyst stage. Immunocytochemistry revealed γH2AX in the one- and four-cell embryos. γH2AX may be involved in repair of preimplantation embryos fertilized with oxygen-stressed spermatozoa.
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影响因子：
6.1
作者：
Morris, ID;Ilott, S;Brison, DR
通讯作者：
Brison, DR
抗坏血酸和过氧化氢酶对人精子冷冻保存的保护作用
影响因子：
--
作者：
Li, Zhiling;Lin, Qionglin;Liu, Wanmin
通讯作者：
Liu, Wanmin
影响因子：
2.5
作者：
Chatterjee, S;Gagnon, C
通讯作者：
Gagnon, C
影响因子：
2.7
作者：
Bucak, Mustafa Numan;Tuncer, Purhan Barbaros;Oztuna, Derya
通讯作者：
Oztuna, Derya
影响因子：
6.1
作者：
Barroso, G;Morshedi, M;Oehninger, S
通讯作者：
Oehninger, S