The effect of erythropoietin on autologous stem cell-mediated bone regeneration.
The effect of erythropoietin on autologous stem cell-mediated bone regeneration.
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DOI：
10.1016/j.biomaterials.2013.06.031
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发表时间：
2013-10
期刊：
影响因子：
14
通讯作者：
Tang, Liping
中科院分区：
文献类型：
--
作者：
Nair, Ashwin M.;Tsai, Yi-Ting;Shah, Krishna M.;Shen, Jinhui;Weng, Hong;Zhou, Jun;Sun, Xiankai;Saxena, Ramesh;Borrelli, Joseph, Jr.;Tang, Liping
关键词：
间充质干细胞（MSC）虽然用于骨组织工程，但受到移植前分离和培养要求的限制。我们最近的研究表明，生物材料植入物可以被工程化，以促进骨髓间充质干细胞的招聘。在这项研究中，我们探讨了这些植入物在骨缺损的情况下直接招募和分化MSCs的能力。我们最初确定，基质衍生因子-1 α（SDF-1α）和促红细胞生成素（Epo）都能促进不同程度的MSC募集。此外，我们发现Epo和骨形态发生蛋白-2（BMP-2），而不是SDF-1α，在体外触发了MSCs的成骨分化。然后，我们研究了使用小鼠颅骨模型指导自体MSC介导的骨再生的可能性。与我们的体外观察一致，发现Epo-释放支架在桥接缺损方面比BMP-2加载支架更有效，如通过计算机断层扫描（CT）扫描、荧光成像和组织学分析所确定的。这些结果证明了巨大的潜力，指导招募和分化的自体骨髓间充质干细胞在组织再生领域。
Mesenchymal stem cells (MSCs) although used for bone tissue engineering are limited by the requirement of isolation and culture prior to transplantation. Our recent studies have shown that biomaterial implants can be engineered to facilitate the recruitment of MSCs. In this study, we explore the ability of these implants to direct the recruitment and the differentiation of MSCs in the setting of a bone defect. We initially determined that both stromal derived factor-1alpha (SDF-1α) and erythropoietin (Epo) prompted different degrees of MSC recruitment. Additionally, we found that Epo and bone morphogenetic protein-2 (BMP-2), but not SDF-1α, triggered the osteogenic differentiation of MSCs in vitro. We then investigated the possibility of directing autologous MSC-mediated bone regeneration using a murine calvaria model. Consistent with our in vitro observations, Epo-releasing scaffolds were found to be more potent in bridging the defect than BMP-2 loaded scaffolds, as determined by Computed Tomography (CT) scanning, fluorescent imaging and histological analyses. These results demonstrate the tremendous potential, directing the recruitment and differentiation of autologous MSCs has in the field of tissue regeneration.
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影响因子：
--
作者：
Kretlow JD;Jin YQ;Liu W;Zhang WJ;Hong TH;Zhou G;Baggett LS;Mikos AG;Cao Y
通讯作者：
Cao Y
DOI：
10.4049/jimmunol.0900803
发表时间：
2009-07-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fiorina P;Jurewicz M;Augello A;Vergani A;Dada S;La Rosa S;Selig M;Godwin J;Law K;Placidi C;Smith RN;Capella C;Rodig S;Adra CN;Atkinson M;Sayegh MH;Abdi R
通讯作者：
Abdi R
影响因子：
2.6
作者：
Covas, Dimas T.;Panepucci, Rodrigo A.;Zago, Marco A.
通讯作者：
Zago, Marco A.
影响因子：
20.3
作者：
GRONTHOS, S;GRAVES, SE;SIMMONS, PJ
通讯作者：
SIMMONS, PJ
影响因子：
4
作者：
Gronthos, S;Zannettino, ACW;Simmons, PJ
通讯作者：
Simmons, PJ