Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.
Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.
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卷心菜 (Brassica oleracea var.capitalata) 是一种富含芥子油苷 (GSL) 的蔬菜，已被证明对健康有益。为了深入了解卷心菜中 GSL 的合成，我们系统地分析了整个卷心菜基因组中的 GSL 生物合成基因 (GBG)。总共鉴定出 193 个卷心菜 GBG，与拟南芥中的 106 个 GBG 同源。卷心菜中的大多数 GBG 都经历了负选择。卷心菜和大白菜中的许多同源GBG在表达模式上存在差异，表明这些同源GBG具有独特的功能。喷洒五种外源激素显着改变了甘蓝中 GBG 的表达水平。例如，MeJA显着上调侧链延伸基因BoIPMILSU1-1和BoBCAT-3-1以及核心结构构建基因BoCYP83A1和BoST5C-1的表达，而ETH显着抑制侧链延伸基因BoIPMILSU1-1、BoCYP79B2-1和BoMAMI-1以及一些转录因子BoMYB28-1、BoMYB34-1、 BoMYB76-1、BoCYP79B2-1 和 BoMAMI-1。从系统发育上看，CYP83家族以及CYP79B和CYP79F亚家族可能仅参与十字花科植物中的GSL合成。我们在全基因组水平上对卷心菜中 GBG 进行了前所未有的鉴定和分析，为通过基因编辑和过度表达调控 GSL 合成奠定了基础。
Cabbage (Brassica oleracea var. capitata) is a vegetable rich in glucosinolates (GSLs) that have proven health benefits. To gain insights into the synthesis of GSLs in cabbage, we systematically analyzed GSLs biosynthetic genes (GBGs) in the entire cabbage genome. In total, 193 cabbage GBGs were identified, which were homologous to 106 GBGs in Arabidopsis thaliana. Most GBGs in cabbage have undergone negative selection. Many homologous GBGs in cabbage and Chinese cabbage differed in expression patterns indicating the unique functions of these homologous GBGs. Spraying five exogenous hormones significantly altered expression levels of GBGs in cabbage. For example, MeJA significantly upregulated side chain extension genes BoIPMILSU1-1 and BoBCAT-3-1, and the expression of core structure construction genes BoCYP83A1 and BoST5C-1, while ETH significantly repressed the expression of side chain extension genes such as BoIPMILSU1-1, BoCYP79B2-1, and BoMAMI-1, and some transcription factors, namely BoMYB28-1, BoMYB34-1, BoMYB76-1, BoCYP79B2-1, and BoMAMI-1. Phylogenetically, the CYP83 family and CYP79B and CYP79F subfamilies may only be involved in GSL synthesis in cruciferous plants. Our unprecedented identification and analysis of GBGs in cabbage at the genome-wide level lays a foundation for the regulation of GSLs synthesis through gene editing and overexpression.
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影响因子：
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Frerigmann H;Glawischnig E;Gigolashvili T
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