Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence.
Speculation on the possibility for introducing Anopheles stephensi as a species complex: preliminary evidence based on odorant binding protein 1 intron I sequence.
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DOI：
10.1186/s12936-018-2523-y
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发表时间：
2018-10-16
期刊：
影响因子：
3
通讯作者：
Gholizadeh S
中科院分区：
文献类型：
--
作者：
Firooziyan S;Dinparast Djadid N;Gholizadeh S
斯氏按蚊被认为是伊朗、亚洲和最近非洲之角的重要疟疾媒介。最近，Ansteobp1内含子I序列已被引入一个新的分子标记，用于鉴定其生物学形式，包括Mysorensis，中间和类型，使用昆虫菌落标本。在目前的研究中，新的标记能力的生物形式的分子鉴定已评估与安。采自伊朗和阿富汗疟疾流行省份的stephensi标本。DNA提取和PCR扩增后，序列分析和构建的系统发育树显示，模式和中间型分布在伊朗。从阿富汗采集的标本被鉴定为中间型和Mysorensis型。因此，在这两个国家，中间型是同域种。根据Ansteobp1内含子I序列的结果，An.斯氏按蚊可能是包括按蚊在内的按蚊复组新种。stephensi sibling A（type form），A. stephensi同胞B（中间型）和An. stephensi sibling C（mysorensis form）.这是关于An存在的第一次报告。在阿富汗发现了stephensi生物。伊朗将消除该国的疟疾传播，精确的物种识别，特别是复杂物种的识别将有助于预防该国疟疾的死灰复燃，主要是因为常见的按蚊物种区系以及阿富汗，巴基斯坦和伊朗境内的边境疟疾和人口流动。
Anopheles stephensi is considered an important malaria vector in Iran, Asia, and recently in the Horn of Africa. Recently, Ansteobp1 intron I sequence has been introduced a new molecular marker for identification of its biological forms including, mysorensis, intermediate and type, using insectary colony specimens. In the current study, new marker ability in molecular identification of biological forms has been evaluated with An. stephensi specimens collected from Iran and Afghanistan malarious provinces. Following DNA extraction and PCR amplification, sequence analysis and constructed phylogenetic tree revealed that type and intermediate forms are distributed in Iran. The specimens collected from Afghanistan identified as intermediate and mysorensis forms. Therefore, intermediate form is sympatric species in both countries. Based on the results of Ansteobp1 intron I sequences, An. stephensi could be suggested as new Anopheles complex species including An. stephensi sibling A (type form), An. stephensi sibling B (intermediate form) and An. stephensi sibling C (mysorensis form). This is the first report on the presence of An. stephensi biological forms in Afghanistan. Iran is going to eliminate malaria transmission from the country, precise species identification, especially in complex species will be helpful in the prevention of malaria resurgence in the country, mainly because of common fauna of Anopheles species and through border malaria and population movement within Afghanistan, Pakistan, and Iran.
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影响因子：
3
作者：
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通讯作者：
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影响因子：
1.7
作者：
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影响因子：
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影响因子：
2.7
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通讯作者：
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