Tissue Localization and Variation of Major Symbionts in Haemaphysalis longicornis, Rhipicephalus haemaphysaloides, and Dermacentor silvarum in China
Tissue Localization and Variation of Major Symbionts in Haemaphysalis longicornis, Rhipicephalus haemaphysaloides, and Dermacentor silvarum in China
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中国长角血蜱、血蜱扇头蜱和森林革蜱主要共生体的组织定位和变异
DOI：
10.1128/aem.00029-18
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发表时间：
2018-03
影响因子：
4.4
通讯作者：
Wang JW
中科院分区：
文献类型：
--
作者：
Wang Mengfei;Zhong Zhengwei;Wang Jingwen;Zhu Dan;Zhang Yi;Dai Jianfeng;Wang JW
蜱是重要的疾病媒介，因为它们在世界范围内传播各种人类和动物病原体。与蜱虫共同进化的共生体在营养代谢、繁殖力和媒介能力方面给宿主带来了至关重要的好处。虽然在中国已经发现了100多种蜱虫，但关于蜱虫共生的一般信息有限。本研究利用荧光原位杂交技术，对实验室饲养的长角血蜱和血蜱碱根头虫的组织分布进行了观察。我们发现，Coxiella sp.只在长角蜱的马尔比氏小管和卵巢中定植，而立克次体sp.也在血蜱的中肠中定植。对内蒙古地区采集的森林革螨蜱的菌群结构进行了调查，发现科希氏菌、立克次体和假单胞菌是3个优势属。雌雄蜱的菌群组成无显著差异。我们再次分析了Coxiella sp.和立克次体sp.的组织定位，发现它们表现出与血蜱相似的组织趋向性，只是立克次体sp.在银蜱的精子细胞核而不是卵巢中定植。综上所述，科希氏体和立克次体是三种蜱的主要共生体，主要分布在中肠、马尔比氏小管和生殖组织中，但其组织分布随种类和性别的不同而不同。蜱传疾病是一项重大的公共卫生负担，因为它们在世界范围内的发生频率和严重程度都在增加。共生体的存在有助于蜱虫代谢营养物质，促进繁殖力，并影响病原体感染。在中国已发现越来越多的蜱传病原体；然而，对本地蜱虫，特别是蜱虫共生的了解是有限的。本研究分析了实验室饲养的长角血蜱、血蜱碱根头蜱和野外采集的森林革螨组织中柯谢氏菌和立克次体的分布。结果表明，中国3种蜱的科西埃氏杆菌定位模式与其他蜱种相似。我们还在蜱的中肠和精子中发现了以前未定义的立克次体在细胞内的定位。此外，我们证明了共生体的组织趋向性在物种和性别之间是不同的。我们的研究结果为中国本土蜱的组织定位提供了新的见解，并为进一步了解它们的功能和与蜱的共生相互作用铺平了道路。
ABSTRACT Ticks are important disease vectors, as they transmit a variety of human and animal pathogens worldwide. Symbionts that coevolved with ticks confer crucial benefits to their host in nutrition metabolism, fecundity, and vector competence. Although over 100 tick species have been identified in China, general information on tick symbiosis is limited. Here, we visualized the tissue distribution of Coxiella sp. and Rickettsia sp. in lab-reared Haemaphysalis longicornis and Rhipicephalus haemaphysaloides by fluorescent in situ hybridization. We found that Coxiella sp. colonized exclusively the Malpighian tubules and ovaries of H. longicornis, while Rickettsia sp. additionally colonized the midgut of R. haemaphysaloides. We also investigated the population structure of microbiota in Dermacentor silvarum ticks collected from Inner Mongolia, China, and found that Coxiella, Rickettsia, and Pseudomonas are the three dominant genera. No significant difference in microbiota composition was found between male and female D. silvarum ticks. We again analyzed the tissue localization of Coxiella sp. and Rickettsia sp. and found that they displayed tissue tropisms similar to those in R. haemaphysaloides, except that Rickettsia sp. colonized the nuclei of spermatids instead of ovaries in D. silvarum. Altogether, our results suggest that Coxiella sp. and Rickettsia sp. are the main symbionts in the three ticks and reside primarily in midgut, Malpighian tubules, and reproductive tissues, but their tissue distribution varies in association with species and sexes. IMPORTANCE Tick-borne diseases constitute a major public health burden, as they are increasing in frequency and severity worldwide. The presence of symbionts helps ticks to metabolize nutrients, promotes fecundity, and influences pathogen infections. Increasing numbers of tick-borne pathogens have been identified in China; however, knowledge of native ticks, especially tick symbiosis, is limited. In this study, we analyze the distribution of Coxiella sp. and Rickettsia sp. in tissues of laboratory-reared Haemaphysalis longicornis and Rhipicephalus haemaphysaloides and field-collected Dermacentor silvarum. We found that the localization patterns of Coxiella sp. in three Chinese tick species were similar to those of other tick species. We also found a previously undefined intracellular localization of Rickettsia sp. in tick midgut and spermatids. In addition, we demonstrate that tissue tropisms of symbionts vary between species and sexes. Our findings provide new insights into the tissue localization of symbionts in native Chinese ticks and pave the way for further understanding of their functional capabilities and symbiotic interactions with ticks.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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中国北方硬蜱 Haemaphysalis longicornis（螨虫：蜱科）的微生物群落和共生体
影响因子：
3.2
作者：
Liu LM;Liu JN;Liu Z;Yu ZJ;Xu SQ;Yang XH;Li T;Li SS;Guo LD;Liu JZ
通讯作者：
Liu JZ
影响因子：
3.2
作者：
Zhou X;Xia S;Huang JL;Tambo E;Zhuge HX;Zhou XN
通讯作者：
Zhou XN
常规方法鉴定微小扇头蜱肠道菌群及PCR-DGGE分析
影响因子：
2.2
作者：
Xu Xing-Li;Cheng Tian-Yin;Yang Hu;Yan Fen
通讯作者：
Yan Fen
影响因子：
5.1
作者：
M. Watt;P. Hugenholtz;R. White;Kerry Vinall
通讯作者：
M. Watt;P. Hugenholtz;R. White;Kerry Vinall