The Mini Colon Model: a benchtop multi-bioreactor system to investigate the gut microbiome.
The Mini Colon Model: a benchtop multi-bioreactor system to investigate the gut microbiome.
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DOI：
10.1080/19490976.2022.2096993
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发表时间：
2022-01
期刊：
影响因子：
12.2
通讯作者：
Juncker, David
中科院分区：
文献类型：
--
作者：
Jin, Zijie;Ng, Andy;Maurice, Corinne F.;Juncker, David
体外发酵系统允许研究肠道微生物群落，精确控制各种生理参数，同时将混杂因素与人类宿主分离。目前的系统，如SHIME和Robogut，占地面积大，缺乏多路复用，实验吞吐量低。解决这些缺点的替代方案，如微型生物反应器阵列系统，通常依赖于昂贵的专业设备，这阻碍了在实验室中的广泛复制。在这里，我们提出了迷你结肠模型（MiCoMo），一个低成本的台式多生物反应器系统，模拟人体结肠环境与生理相关的条件。该装置由三个生物反应器组成，独立于厌氧室工作，并配备自动pH值、温度和流体控制。我们进行了14天的实验，发现MiCoMo能够在接种3-5天后从单个粪便样品中支持稳定的复杂微生物群群落，香农指数为3.17 ± 0.65。MiCoMo还通过开发每个供体特有的密切相关的群落来保留样品间的微生物差异，同时在这个短暂的稳定期后保持重复反应器之间的最小变化（平均Bray-Curtis相似性0.72 ± 0.13）和每日变化（平均Bray-Curtis相似性0.81±0.10）。总之，这些结果建立了MiCoMo作为一个可访问的系统，用于研究具有高通量和多路复用能力的肠道微生物群落。
In vitro fermentation systems allow for the investigation of gut microbial communities with precise control of various physiological parameters while decoupling confounding factors from the human host. Current systems, such as the SHIME and Robogut, are large in footprint, lack multiplexing, and have low experimental throughput. Alternatives which address these shortcomings, such as the Mini Bioreactor Array system, are often reliant on expensive specialized equipment, which hinders wide replication across labs. Here, we present the Mini Colon Model (MiCoMo), a low-cost, benchtop multi-bioreactor system that simulates the human colon environment with physiologically relevant conditions. The device consists of triplicate bioreactors working independently of an anaerobic chamber and equipped with automated pH, temperature, and fluidic control. We conducted 14-d experiments and found that MiCoMo was able to support a stable complex microbiota community with a Shannon Index of 3.17 ± 0.65, from individual fecal samples after only 3–5 d of inoculation. MiCoMo also retained inter-sample microbial differences by developing closely related communities unique to each donor, while maintaining both minimal variations between replicate reactors (average Bray-Curtis similarity 0.72 ± 0.13) andday-to-day variations (average Bray-Curtis similarity 0.81±0.10) after this short stabilization period. Together, these results establish MiCoMo as an accessible system for studying gut microbial communities with high throughput and multiplexing capabilities.
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影响因子：
6.1
作者：
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影响因子：
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DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob