Borrelia burgdorferi and Borrelia miyamotoi seroprevalence in California blood donors.
Borrelia burgdorferi and Borrelia miyamotoi seroprevalence in California blood donors.
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DOI：
10.1371/journal.pone.0243950
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Smith WA
中科院分区：
文献类型：
--
作者：
Brummitt SI;Kjemtrup AM;Harvey DJ;Petersen JM;Sexton C;Replogle A;Packham AE;Bloch EM;Barbour AG;Krause PJ;Green V;Smith WA
西部黑腿蜱，太平洋硬蜱，是美国西部两种人畜共患螺旋体的重要媒介：伯氏疏螺旋体（也称为伯氏疏螺旋体），引起莱姆病，和宫本疏螺旋体，引起复发性发热型疾病。在加州，莱姆病的人类病例有很好的记录，在北部沿海地区和内华达州山脉的西坡，风险增加。尽管B已经存在。在食人岛的宫本井。在加州发现的太平洋蜱中，这种螺旋体的临床病例尚未得到充分研究。评估B暴露。burgdorferi和B. miyamotoi在加州，并解决假设，B。人类的miyamotoi暴露在地理范围内与B相似。在burgdorferi中，对来自加州的1,700份献血者血清进行了针对两种病原体的抗体测试。样本来自加州莱姆病高流行和低流行县。使用C6 ELISA筛选所有血清。进一步分析所有C6阳性和可疑样本以及9份随机选择的C6阴性样本的B。用IgG蛋白质印迹法和改良的两种ELISA检测系统检测伯氏螺旋体抗体和B。miyamotoi抗体，使用GlpQ ELISA和B.宫本全细胞超声蛋白质印迹法。在1,700个系列测试样本中，有8个样本对B抗体呈阳性。burgdorferi（0.47%，精确95% CI：0.20，0.93）和2例B抗体检测阳性。miyamotoi（0.12%，精确95% CI：0.01，0.42）。莱姆病高、低流行县两种病原体血清阳性率差异无统计学意义。我们的研究结果证实了莱姆病的低频率和B的频率甚至更低。加州成年献血者中的miyamotoi暴露;然而，我们的研究结果强化了公共卫生信息，即该州存在这些新兴疾病感染的风险。
The western blacklegged tick, Ixodes pacificus, an important vector in the western United States of two zoonotic spirochetes: Borrelia burgdorferi (also called Borreliella burgdorferi), causing Lyme disease, and Borrelia miyamotoi, causing a relapsing fever-type illness. Human cases of Lyme disease are well-documented in California, with increased risk in the north coastal areas and western slopes of the Sierra Nevada range. Despite the established presence of B. miyamotoi in the human-biting I. pacificus tick in California, clinical cases with this spirochete have not been well studied. To assess exposure to B. burgdorferi and B. miyamotoi in California, and to address the hypothesis that B. miyamotoi exposure in humans is similar in geographic range to B. burgdorferi, 1,700 blood donor sera from California were tested for antibodies to both pathogens. Sampling was from high endemic and low endemic counties for Lyme disease in California. All sera were screened using the C6 ELISA. All C6 positive and equivocal samples and nine randomly chosen C6 negative samples were further analyzed for B. burgdorferi antibody using IgG western blot and a modified two ELISA test system and for B. miyamotoi antibody using the GlpQ ELISA and B. miyamotoi whole cell sonicate western blot. Of the 1,700 samples tested in series, eight tested positive for antibodies to B. burgdorferi (0.47%, Exact 95% CI: 0.20, 0.93) and two tested positive for antibodies to B. miyamotoi (0.12%, Exact 95% CI: 0.01, 0.42). There was no statistically significant difference in seroprevalence for either pathogen between high and low Lyme disease endemic counties. Our results confirm a low frequency of Lyme disease and an even lower frequency of B. miyamotoi exposure among adult blood donors in California; however, our findings reinforce public health messaging that there is risk of infection by these emerging diseases in the state.
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DOI：
10.4269/ajtmh.2006.74.632
发表时间：
2006-04-01
影响因子：
3.3
作者：
Eisen, RJ;Eisen, L;Lane, RS
通讯作者：
Lane, RS
影响因子：
14.2
作者：
Koetsveld, J.;Platonov, A. E.;Hovius, J. W.
通讯作者：
Hovius, J. W.
影响因子：
9.4
作者：
Bacon, RM;Pilgard, MA;Schwan, TG
通讯作者：
Schwan, TG
影响因子：
4
作者：
Jahfari, S;Herremans, T;Platonov, A E;Kuiper, H;Karan, L S;Vasilieva, O;Koopmans, M P G;Hovius, J W R;Sprong, H
通讯作者：
Sprong, H
影响因子：
11.8
作者：
Branda, John A.;Linskey, Katy;Ferraro, Mary Jane
通讯作者：
Ferraro, Mary Jane