Hydrogen cyanide produced by the soil bacterium Chromobacterium sp. Panama contributes to mortality in Anopheles gambiae mosquito larvae.
Hydrogen cyanide produced by the soil bacterium Chromobacterium sp. Panama contributes to mortality in Anopheles gambiae mosquito larvae.
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DOI：
10.1038/s41598-018-26680-2
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发表时间：
2018-05-29
影响因子：
4.6
通讯作者：
Dimopoulos G
中科院分区：
文献类型：
--
作者：
Short SM;van Tol S;MacLeod HJ;Dimopoulos G
苔藓虫幼虫在其水生环境中不断遇到微生物，这些微生物作为食物并在成功发育中发挥关键作用。在前期工作中，我们从巴拿马登革热病媒伊蚊中肠分离到一株具有杀幼虫活性的色杆菌（C.sp_P）。在本研究中，我们发现C.sp_P的初始浓度与幼虫死亡率之间呈正相关，并且C.sp_P在高营养环境中更有效地诱导幼虫死亡。多种色杆菌引起的幼虫死亡率与C.sp_P相似，但C.次松我们还发现，非致死剂量的C.sp_P延长了发育时间，并增加了多个发育阶段的死亡率，表明暴露的持续影响。此外，我们表明，杀幼虫活性在去除活细菌后的幼虫养殖水中持续存在，并且C.sp_P处理的水中的杀幼虫因子小于3kDa，耐热至90 °C，并且在真空离心后损失。我们发现，C.sp_P在培养物和幼虫水中产生的氰化氢浓度足以杀死An。冈比亚幼虫，用氰化物解毒剂处理幼虫水消除了杀幼虫活性。因此，C.sp_P诱导幼虫死亡的一个可能机制是通过产生氰化氢。
Mosquito larvae continuously encounter microbes in their aquatic environment, which serve as food and play a critical role in successful development. In previous work, we isolated a Chromobacterium sp. (C.sp_P) with larvicidal activity from the midgut of dengue vector Aedes mosquitoes in Panama. In this study, we found a positive correlation between initial concentrations of C.sp_P and larval mortality rates, and that C.sp_P is more efficient at inducing larval mortality in a high nutrient environment. Multiple Chromobacterium species induce larval mortality with similar efficacy to C.sp_P except for C. subtsugae. We also found that a non-lethal dose of C.sp_P lengthens development time and increases mortality over multiple developmental stages, suggesting persistent effects of exposure. Additionally, we showed that larvicidal activity persists in the larval breeding water after removal of live bacteria, and that the larvicidal factor in C.sp_P-treated water is smaller than 3 kDa, heat resistant to 90 °C, and lost after vacuum centrifugation. We showed that C.sp_P produces hydrogen cyanide in culture and in larval water at concentrations sufficient to kill An. gambiae larvae, and treatment of the larval water with a cyanide antidote eliminated larvicidal activity. We conclude that a potential mechanism by which C.sp_P can induce larval mortality is via production of hydrogen cyanide.
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影响因子：
3.7
作者：
Apte-Deshpande A;Paingankar M;Gokhale MD;Deobagkar DN
通讯作者：
Deobagkar DN
影响因子：
3.7
作者：
Akorli J;Gendrin M;Pels NA;Yeboah-Manu D;Christophides GK;Wilson MD
通讯作者：
Wilson MD
DOI：
10.1039/j19690000381
发表时间：
1969-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY A -INORGANIC PHYSICAL THEORETICAL
影响因子：
--
作者：
FIRTH, RA;HILL, HAO;WILLIAMS, RJP
通讯作者：
WILLIAMS, RJP
影响因子：
2.8
作者：
CASTRIC, PA
通讯作者：
CASTRIC, PA
影响因子：
6.4
作者：
Broderick, Kate E.;Chan, Adriano;Boss, Gerry R.
通讯作者：
Boss, Gerry R.