Determination of cross sections by overtone mobility spectrometry: evidence for loss of unstable structures at higher overtones.
Determination of cross sections by overtone mobility spectrometry: evidence for loss of unstable structures at higher overtones.
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DOI：
10.1021/jp1060123
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发表时间：
2010-09-30
影响因子：
3.3
通讯作者：
Clemmer, David E.
中科院分区：
文献类型：
--
作者：
Lee, Sunyoung;Ewing, Michael A.;Nachtigall, Fabiane M.;Kurulugama, Ruwan T.;Valentine, Stephen J.;Clemmer, David E.
泛音迁移谱 (OMS) 是一种确定多电荷泛素和物质 P 离子以及单电荷棉子糖和松三糖离子碰撞截面的方法。总体而言，通过 OMS 测量的稳定离子构象的碰撞截面值与传统离子迁移谱 (IMS) 测量确定的值一致，相对不确定度在 1% 以内。泛素离子的 OMS 谱似乎有利于更高泛音的不同构象。我们提出，分布的变化作为测量泛音区域的函数，是由于消除了在漂移区域中经历结构转变的离子而引起的。动力学模拟表明，OMS 测量可检测到发生在几毫秒数量级的结构转变，并导致离子碰撞截面发生约 4% 的变化。利用 OMS 区分离子淌度的独特方法揭示了传统 IMS 测量中不易察觉的结构转变。
Overtone mobility spectrometry (OMS) is examined as a means of determining the collision cross sections for multiply-charged ubiquitin and substance P ions, as well as for singly-charged rafinose and melezitose ions. Overall, values of collision cross section measured by OMS for stable ion conformations are found to be in agreement with values determined by conventional ion mobility spectrometry (IMS) measurements to within ~1%, relative uncertainty. The OMS spectra for ubiquitin ions appear to favor different conformations at higher overtones. We propose that the changes in the distributions as a function of the overtone region in which they are measured arise from the elimination of ions that undergo structural transitions in the drift regions. Kinetics simulations suggest that structural transitions occurring on the order of a few ms and resulting in a ~4% change in ion collision cross sections are detected by OMS measurements. The unique method of distinguishing ion mobilities with OMS reveals these structural transitions which are not readily apparent from traditional IMS measurements.
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影响因子：
1.8
作者：
Gill, AC;Jennings, KR;Bowers, MT
通讯作者：
Bowers, MT
影响因子：
7.4
作者：
Badman, ER;Hoaglund-Hyzer, CS;Clemmer, DE
通讯作者：
Clemmer, DE
影响因子：
3.3
作者：
Julian, RR;Myung, S;Clemmer, DE
通讯作者：
Clemmer, DE
DOI：
10.1016/1044-0305(93)85025-s
发表时间：
1993-08-01
影响因子：
3.2
作者：
COVEY, T;DOUGLAS, DJ
通讯作者：
DOUGLAS, DJ
影响因子：
7.4
作者：
Hoaglund, CS;Valentine, SJ;Clemmer, DE
通讯作者：
Clemmer, DE