The gut microbiota profile is associated with insulin action in humans.
The gut microbiota profile is associated with insulin action in humans.
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DOI：
10.1007/s00592-012-0410-5
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发表时间：
2013-10
影响因子：
3.8
通讯作者：
Tinahones, Francisco
中科院分区：
文献类型：
--
作者：
Serino, Matteo;Manuel Fernandez-Real, Jose;Fuentes, Eduardo Garcia;Queipo-Ortuno, Maribel;Maria Moreno-Navarrete, Jose;Sanchez, Alex;Burcelin, Remy;Tinahones, Francisco
肠道微生物群在诱导代谢性疾病中的作用现在已在全球范围内得到越来越多的认可。事实上，特定的肠道微生物群已被证明可以表征人类和小鼠中的瘦型与肥胖型表型。我们最近还证明，精确的肠道微生物群与宿主对高脂肪饮食的反应有关。因此，我们假设人类的胰岛素抵抗也可能与特定的肠道微生物群有关。为此，从胰岛素抵抗与胰岛素敏感性肥胖受试者的阑尾内容物中提取微生物DNA和RNA，匹配体重指数和年龄，并通过DNA和RNA-DGGE进行分析。微生物DNA分析表明，患者完全根据他们的胰岛素作用程度而分离。相反，微生物RNA研究表明，胰岛素敏感和胰岛素抵抗患者之间仍然存在一定程度的同源性。数量性状分析、普通最小二乘回归、主成分回归、偏最小二乘、典型相关分析和典型对应分析也显示了两种分析表型的净分离。我们的结论是，一个特定的肠道微生物的档案与胰岛素的行动在人类。
The role of the gut microbiota in the induction of metabolic diseases has now been increasingly recognized worldwide. Indeed, a specific gut microbiota has been shown to characterize lean versus obese phenotypes both in humans and mice. We have also recently demonstrated that a precise gut microbiota is associated with the host’s responsiveness to a high-fat diet. Therefore, we hypothesized that insulin resistance in humans could also be linked to a specific gut microbiota. To this aim, microbial DNA and RNA were extracted from the appendix contents of insulin-resistant versus insulin-sensitive obese subjects, matched for body mass index and age, and analyzed by DNA- and RNA-DGGE. Microbial DNA analysis showed that the patients fully segregated according to their degree of insulin action. Conversely, microbial RNA investigation showed that some degree of homology still existed between insulin-sensitive and insulin-resistant patients. Quantitative trait analysis, ordinary least squares regression, principal components regression, partial least squares, canonical correlation analysis, and canonical correspondence analysis also showed a net separation of the two phenotypes analyzed. We conclude that a specific gut microbial profile is associated with insulin action in humans.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
影响因子：
16.2
作者：
Moreno-Navarrete JM;Fernández-Real JM
通讯作者：
Fernández-Real JM
影响因子：
3.1
作者：
Burcelin, Remy;Luche, Elodie;Amar, Jacques
通讯作者：
Amar, Jacques
DOI：
10.1196/annals.1309.015
发表时间：
2004-01-01
期刊：
ORAL TOLERANCE: NEW INSIGHTS AND PROSPECTS FOR CLINICAL APPLICATION
影响因子：
--
作者：
Gebbers, JO;Laissue, JA
通讯作者：
Laissue, JA
影响因子：
24.5
作者：
Serino M;Luche E;Gres S;Baylac A;Bergé M;Cenac C;Waget A;Klopp P;Iacovoni J;Klopp C;Mariette J;Bouchez O;Lluch J;Ouarné F;Monsan P;Valet P;Roques C;Amar J;Bouloumié A;Théodorou V;Burcelin R
通讯作者：
Burcelin R