Neuromuscular transmitter candidates of a centipede (Lithobius forficatus, Chilopoda).
Neuromuscular transmitter candidates of a centipede (Lithobius forficatus, Chilopoda).
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DOI：
10.1186/s12983-018-0274-9
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发表时间：
2018
影响因子：
2.8
通讯作者：
Stern M
中科院分区：
文献类型：
--
作者：
Langeloh H;Wasser H;Richter N;Bicker G;Stern M
神经肌肉接头是运动神经元与骨骼肌纤维通讯的化学突触。尽管脊椎动物和许多无脊椎动物在神经肌肉接头处使用乙酰胆碱作为递质，但到目前为止，在这些节肢动物中，谷氨酸和GABA被使用。然而，关于系统发育背景下的分类单元采样，只有有限的数据，主要集中在甲壳类和六足类，而忽略了其他节肢动物群体。在这里，我们调查的多足动物，石forficatus神经肌肉突触的神经递质设备，使用免疫荧光和组织化学染色方法。谷氨酸和γ-氨基丁酸与突触蛋白共定位于石首鱼体壁和腿肌的突触结。乙酰胆碱酯酶活性作为胆碱能突触的标记物，在中枢神经系统和某些外周神经中大量发现，但在神经肌肉接头中没有发现。此外，大量的腿感觉神经元显示GABA-免疫荧光，也标记了抗血清对GABA合成酶，谷氨酸脱羧酶。我们的数据表明，谷氨酸和GABA的神经递质在石首鱼forficatus神经肌肉接头，而乙酰胆碱是非常不可能在这里发挥作用。这符合谷氨酸作为真节足动物中兴奋性神经肌肉递质和GABA作为主要抑制性神经肌肉递质的概念。此外，我们有，我们的知识第一次，本地化GABA真节肢动物腿部感觉神经元，表明节肢动物感觉系统的神经递质面板可能远远超过迄今为止假设的可能性。
The neuromuscular junction is the chemical synapse where motor neurons communicate with skeletal muscle fibers. Whereas vertebrates and many invertebrates use acetylcholine as transmitter at the neuromuscular junction, in those arthropods examined up to now, glutamate and GABA are used instead. With respect to taxon sampling in a phylogenetic context, there is, however, only a limited amount of data available, focusing mainly on crustaceans and hexapods, and neglecting other, arthropod groups. Here we investigate the neurotransmitter equipment of neuromuscular synapses of a myriapod, Lithobius forficatus, using immunofluorescence and histochemical staining methods. Glutamate and GABA could be found colocalised with synapsin in synaptic boutons of body wall and leg muscles of Lithobius forficatus. Acetylcholinesterase activity as a marker for cholinergic synapses was found abundantly in the central nervous system and also in some peripheral nerves, but not at neuromuscular junctions. Furthermore, a large number of leg sensory neurons displayed GABA-immunofluorescence and was also labeled with an antiserum against the GABA-synthesizing enzyme, glutamate decarboxylase. Our data indicate that glutamate and GABA are neurotransmitters at Lithobius forficatus neuromuscular junctions, whereas acetylcholine is very unlikely to play a role here. This is in line with the concept of glutamate as excitatory and GABA as the main inhibitory neuromuscular transmitters in euarthropods. Furthermore, we have, to our knowledge for the first time, localized GABA in euarthropod leg sensory neurons, indicating the possibility that neurotransmitter panel in arthropod sensory systems may be far more extensive than hitherto assumed.
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影响因子：
5.5
作者：
BARKER, DL;KRAVITZ, EA;HERBERT, E
通讯作者：
HERBERT, E
影响因子：
4.4
作者：
Battaglioli, G;Liu, HC;Martin, DL
通讯作者：
Martin, DL
DOI：
10.1002/neu.480280406
发表时间：
1995-12-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
LEITCH, B;PITMAN, RM
通讯作者：
PITMAN, RM
影响因子：
2.4
作者：
Martin, RJ;Valkanov, MA;Murray, I
通讯作者：
Murray, I
影响因子：
3.6
作者：
Csoknya, M;Takács, B;Elekes, K
通讯作者：
Elekes, K